Genetic Variation of Capsid Protein VP7 in Genotype G4 Human Rotavirus Strains: Simultaneous Emergence and Spread of Different Lineages in Argentina
Genetic Variation of Capsid Protein VP7 in Genotype G4 Human Rotavirus Strains: Simultaneous Emergence and Spread of Different Lineages in Argentina
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基因型 G4 人轮状病毒株衣壳蛋白 VP7 的遗传变异：不同谱系在阿根廷的同时出现和传播
DOI：
10.1128/jcm.40.6.2016-2022.2002
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发表时间：
2002
影响因子：
9.4
通讯作者：
Jorge A. Gomez
中科院分区：
文献类型：
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作者：
K. Bok;D. Matson;Jorge A. Gomez
摘要 轮状病毒是幼儿严重腹泻的最常见原因。完整的轮状病毒表征包括确定两种外衣壳蛋白 VP7 和 VP4（分别指定为 G 型和 P 型）的抗原类型。在一项全国性轮状病毒监测研究中，G4 基因型频率在第二年有所增加。为了进一步评估G4菌株的出现机制和相互关系，对这些样品中VP7衣壳蛋白的遗传多样性进行了详细研究。总体核苷酸序列差异范围为小于 0.1% 至 19.5%，比其他轮状病毒 G 型观察到的差异（0.1% 至 9%）更高。根据核苷酸距离，序列被分为两个主要谱系（指定为 I 和 II）。最异质的谱系进一步细分为四个亚谱系（指定为 Ia 至 Id）。大多数阿根廷序列属于Ib和Ic亚系，简约分析证实它们是独立的序列簇。这项研究描述了 G4 菌株内的不同谱系和亚谱系，并表明阿根廷菌株与参考菌株 ST3 具有远亲关系。 1998 年期间至少出现两个 G4 基因型（亚）谱系表明这些菌株频率的增加是由于不同菌株组的同步出现。
ABSTRACT Rotavirus is the most-common cause of severe diarrhea in young children. Complete rotavirus characterization includes determination of the antigenic type of the two outer capsid proteins, VP7 and VP4, designated G and P types, respectively. During a nationwide rotavirus surveillance study, genotype G4 frequency increased during the second year. To evaluate further the mechanism of emergence and the relationship among G4 strains, the genetic diversity of VP7 capsid protein in these samples was studied in detail. Overall nucleotide sequence divergence ranged from less than 0.1 to 19.5%, a higher divergence than that observed for other rotavirus G types (0.1 to 9%). Sequences were classified into two major lineages (designated I and II) based on their nucleotide distances. The most heterogeneous lineage was further subdivided into four sublineages (designated Ia to Id). Most Argentine sequences were of sublineages Ib and Ic, which were confirmed to be independent sequence clusters by parsimony analysis. This study describes different lineages and sublineages within G4 strains and shows that Argentine strains are distantly related to reference strain ST3. The appearance of at least two G4 genotype (sub)lineages during 1998 demonstrates that the increased frequency of these strains was due to the synchronized emergence of different groups of strains.
Matsumoto 等人：“副溶血性弧菌 O3:K6 克隆的大流行传播以及相关菌株的出现，由任意引物 PCR 和 toxRS 序列分析证明”J.
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K.Taniguchi：“VP1 和 VP7 基因的序列分析表明，存在导致胃肠炎流行的 G2 轮状病毒重配体。”
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