mRNA overexpression of the hypoxia inducible factor 1 alpha subunit gene (HIF1A): An independent predictor of poor overall survival in chronic lymphocytic leukemia
mRNA overexpression of the hypoxia inducible factor 1 alpha subunit gene (HIF1A): An independent predictor of poor overall survival in chronic lymphocytic leukemia
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DOI：
10.1016/j.leukres.2016.11.014
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发表时间：
2017-02-01
影响因子：
2.7
通讯作者：
Pappa, Vasiliki
中科院分区：
文献类型：
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作者：
Kontos, Christos K.;Papageorgiou, Sotirios G.;Pappa, Vasiliki
缺氧诱导因子 1 (HIF1) 是一种异二聚体转录因子，最终调节细胞对氧张力变化的反应。在这项研究中，我们检查了 HIF1 调节 α 亚基 (HIF1A) mRNA 表达在慢性淋巴细胞白血病 (CLL) 中的潜在诊断和预后潜力。为此，从 88 名 CLL 患者和 33 名非白血病献血者收集的外周血单核细胞中分离出总 RNA，并对聚 (A)-RNA 进行反转录。使用实时 PCR 定量 HIF1A mRNA 水平。 Kaplan-Meier 生存分析表明，HIF1A mRNA 高表达预示 CLL 患者总体生存较差 (p=0.001)。 Bootstrap 单变量 Cox 回归分析证实，HIF1A mRNA 过表达是 CLL 的显着不利预后因子（风险比 = 3.75，偏倚校正和加速 95% 置信区间 = 1.43-24.36，bootstrap p
The hypoxia inducible factor 1 (HIF1) is a heterodimeric transcription factor that ultimately regulates cellular responses to changes in oxygen tension. In this study, we examined the potential diagnostic and prognostic potential of the mRNA expression of HIF1 regulatory alpha-subunit (HIF1A) in chronic lymphocytic leukemia (CLL). For this purpose, total RNA was isolated from peripheral blood mononuclear cells collected from 88 CLL patients and 33 non-leukemic blood donors, and poly(A)-RNA was reversely transcribed. HIF1A mRNA levels were quantified using real-time PCR. Kaplan-Meier survival analysis showed that high HIF1A mRNA expression predicts inferior overall survival for CLL patients (p=0.001). Bootstrap univariate Cox regression analysis confirmed that HIF1A mRNA overexpression is a significant unfavorable prognosticator in CLL (hazard ratio=3.75, bias-corrected and accelerated 95% confidence interval=1.43-24.36, bootstrap p