Isolation and preliminary characterization of a new pathogenic iridovirus from redclaw crayfish Cherax quadricarinatus
Isolation and preliminary characterization of a new pathogenic iridovirus from redclaw crayfish Cherax quadricarinatus
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红螯螯虾新致病性虹彩病毒的分离及初步鉴定
DOI：
10.3354/dao03007
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发表时间：
2016-06-15
影响因子：
1.4
通讯作者：
Yang, Feng
中科院分区：
文献类型：
--
作者：
Xu, Limei;Wang, Tiantian;Yang, Feng
我们报告了从中国福建一个农场采集的患病红螯虾中检测到的一种新虹彩病毒的初步特征。透射电子显微镜发现造血组织细胞的细胞质和质膜上有大量二十面体颗粒（直径约150 nm）。从垂死的 C.quadricarinatus 的血淋巴中半纯化的病毒体的 SDS-PAGE 鉴定出 24 种蛋白质，包括 50 kDa 主要衣壳蛋白 (MCP)。通过对 DNA 限制性内切酶片段的大小进行求和，病毒基因组的长度估计约为 150 kb。使用针对虹彩病毒和氯虹彩病毒中保守的 MCP 基因区域的简并引物通过 PCR 扩增的 103 bp MCP 基因区域推导出的 34 个氨基酸序列与 Sergestid 虹彩病毒最相似（55% 同一性）。根据病毒体形态、蛋白质组成、DNA基因组长度和MCP序列相关性，鉴定出的病毒暂定名为红螯虾虹彩病毒（CQIV）。此外，健康的红虾、克氏原螯虾和凡纳滨对虾实验性感染CQIV也引起了相同的疾病和高死亡率，表明CQIV对养殖和野生小龙虾和虾构成潜在威胁。
We report the preliminary characterization of a new iridovirus detected in diseased Cherax quadricarinatus collected from a farm in Fujian, China. Transmission electron microscopy identified numerous icosahedral particles (similar to 150 nm in diameter) in the cytoplasm and budding from the plasma membrane of hematopoietic tissue cells. SDS-PAGE of virions semi-purified from the hemolymph of moribund C. quadricarinatus identified 24 proteins including a 50 kDa major capsid protein (MCP). By summing the sizes of DNA restriction endonuclease fragments, the viral genome was estimated to be similar to 150 kb in length. A 34 amino acid sequence deduced from a 103 bp MCP gene region amplified by PCR using degenerate primers targeted to MCP gene regions conserved among iridoviruses and chloriridoviruses was most similar (55% identity) to Sergestid iridovirus. Based on virion morphology, protein composition, DNA genome length, and MCP sequence relatedness, the virus identified has tentatively been named Cherax quadricarinatus iridovirus (CQIV). In addition, experimental infection of healthy C. quadricarinatus, Procambarus clarkii, and Litopenaeus vannamei with CQIV caused the same disease and high mortality, suggesting that CQIV poses a potential threat to cultured and wild crayfish and shrimp.