Chloroplast NADPH-Thioredoxin Reductase Interacts with Photoperiodic Development in Arabidopsis
Chloroplast NADPH-Thioredoxin Reductase Interacts with Photoperiodic Development in Arabidopsis
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DOI：
10.1104/pp.108.133777
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发表时间：
2009-03-01
期刊：
影响因子：
7.4
通讯作者：
Rintamaki, Eevi
中科院分区：
文献类型：
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作者：
Lepisto, Anna;Kangasjarvi, Saijaliisa;Rintamaki, Eevi
叶绿体NADPH-硫氧还蛋白还原酶(NTRC)属于硫氧还蛋白系统，控制植物体内关键的代谢和调控途径。在这里，通过对NTRC基因T-DNA插入线的鉴定，我们发现了拟南芥(Arabiopsis Thaliana)叶绿体硫醇氧化还原调控和光周期生长控制之间的新联系。转录本和代谢物图谱显示，NTRC植物在短短8小时的光照下存在严重的发育和代谢缺陷。除了降低叶绿素和花青素的含量外，NTRC植物的氨基酸和生长素水平也发生了变化。此外，NTRC叶片的低碳同化率与蒸腾作用和光呼吸作用的增强有关。当植物生长在长达16小时的光周期下时，NTRC的所有这些特征都不那么严重。转录谱分析表明，NTRC的突变表型伴随着与NTRC植物的气孔发育、叶绿素生物合成、叶绿体生物发生和昼夜时钟连接的光感知系统相关的基因的差异表达。我们认为，NTRC调控叶绿体中的几个关键过程，包括叶绿素生物合成和莽草酸途径。在缺乏NTRC的情况下，不平衡的代谢活动可能调节叶绿体逆行信号，导致核基因表达改变，最终导致NTRC突变植物中多营养表型的形成。
Chloroplast NADPH-thioredoxin reductase (NTRC) belongs to the thioredoxin systems that control crucial metabolic and regulatory pathways in plants. Here, by characterization of T-DNA insertion lines of NTRC gene, we uncover a novel connection between chloroplast thiol redox regulation and the control of photoperiodic growth in Arabidopsis (Arabidopsis thaliana). Transcript and metabolite profiling revealed severe developmental and metabolic defects in ntrc plants grown under a short 8-h light period. Besides reduced chlorophyll and anthocyanin contents, ntrc plants showed alterations in the levels of amino acids and auxin. Furthermore, a low carbon assimilation rate of ntrc leaves was associated with enhanced transpiration and photorespiration. All of these characteristics of ntrc were less severe when plants were grown under a long 16-h photoperiod. Transcript profiling revealed that the mutant phenotypes of ntrc were accompanied by differential expression of genes involved in stomatal development, chlorophyll biosynthesis, chloroplast biogenesis, and circadian clock-linked light perception systems in ntrc plants. We propose that NTRC regulates several key processes, including chlorophyll biosynthesis and the shikimate pathway, in chloroplasts. In the absence of NTRC, imbalanced metabolic activities presumably modulate the chloroplast retrograde signals, leading to altered expression of nuclear genes and, ultimately, to the formation of the pleiotrophic phenotypes in ntrc mutant plants.