USE OF RYANODINE FOR FUNCTIONAL REMOVAL OF THE CALCIUM STORE IN SMOOTH-MUSCLE CELLS OF THE GUINEA-PIG
USE OF RYANODINE FOR FUNCTIONAL REMOVAL OF THE CALCIUM STORE IN SMOOTH-MUSCLE CELLS OF THE GUINEA-PIG
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DOI：
10.1016/s0006-291x(88)80730-7
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发表时间：
1988-04-15
影响因子：
3.1
通讯作者：
ENDO, M
中科院分区：
文献类型：
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作者：
IINO, M;KOBAYASHI, T;ENDO, M
豚鼠门静脉、肺动脉和盲肠带绦虫皮肤纤维的钙库分为两类：一类既有钙诱导的钙释放(CICR)机制，又有1，4，5-三磷酸(IP3)诱导的钙释放(IICR)机制。另一组仅有IICR机制(S.beta)。在CICR激活期间应用兰尼定可锁定CICR通道，但该药物对IICR机制几乎没有影响。因此，在Ryanodine处理后，Ca与CICR(S.α.)失去了容纳钙的能力。由于兰诺定治疗，激动剂引起的完整肌肉收缩的变化表明激动剂从S.pha中释放钙。这会导致肌挛缩的初始阶段。
Calcium store of the skinned fibers of the guinea-pig portal vein, pulmonary artery and taenia caeci consisted of two classes: one with both Ca-induced Ca release (CICR) and inositol 1,4,5-trisphosphate (IP3)-induced Ca release (IICR) mechanisms (S.alpha.) and the other only with IICR mechanism (S.beta.). Ryanodine, applied during the CICR was activated, locked the CICR channels open, but the drug had practically no effect on the IICR mechanism. Thus, after the ryanodine treatment the Ca store with the CICR (S.alpha.) lost its capacity to hold Ca. Changes in the agonist-evoked contraction of intact muscle due to the ryanodine treatment suggest that agonists release Ca from S.alpha. which produces the initial phase of contractures.