Techniques to Assess Phage-Biofilm Interaction
Techniques to Assess Phage-Biofilm Interaction
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DOI：
10.1007/978-1-4939-8940-9_11
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Azeredo, Joana
中科院分区：
文献类型：
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作者：
Boas, Diana Vilas;Almeida, Carina;Azeredo, Joana
生物膜在自然界中无处不在，几乎存在于每一种有生命的和惰性的表面。它们基本上由附着在表面上的微生物组成，周围是由细胞外聚合物质自产的基质。噬菌体已被证明在控制生物膜方面是成功的。在这里，我们描述了表征噬菌体-生物膜相互作用的方法，特别是评估生物膜生物量和可视化生物膜结构，使用靶向分子探针区分感染细胞。
Biofilms are ubiquitous in nature found on nearly every type of living and inert surface. They basically consist of microorganisms attached to surfaces and surrounded by a self-produced matrix of extracellular polymeric substances. Phages have proven to be successful in controlling biofilms. Here, we describe methods to characterize phage-biofilm interactions, specifically to assess biofilm biomass and to visualize the biofilm structure, discriminating infected cells using targeted molecular probes.