Biotransformation of (+)-catechin into taxifolin by a two-step oxidation:: Primary stage of (+)-catechin metabolism by a novel (+)-catechin-degrading bacteria, Burkholderia sp KTC-1, isolated from tropical peat
Biotransformation of (+)-catechin into taxifolin by a two-step oxidation:: Primary stage of (+)-catechin metabolism by a novel (+)-catechin-degrading bacteria, Burkholderia sp KTC-1, isolated from tropical peat
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DOI：
10.1016/j.bbrc.2007.11.157
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发表时间：
2008-02-08
影响因子：
3.1
通讯作者：
Osaki, Mitsuru
中科院分区：
文献类型：
--
作者：
Matsuda, Motoki;Otsuka, Yuichiro;Osaki, Mitsuru
泥炭含有从植物材料中提取的各种持久性化合物。我们分离到一株新型(+)-儿茶素降解菌Burkholderia sp.KTC-1(KTC-1)，作为能够降解热带泥炭中存在的持久性芳香化合物的细菌的例子。该菌以(+)-儿茶素为唯一碳源在酸性条件下生长。粗酶提取物的反应及其产物的结构研究表明，(+)-儿茶素在其代谢的初始阶段被KTC-1生物转化为紫杉林。高效液相色谱分析表明，这一转变发生在两个氧化步骤：4-羟基化和脱氢。此外，(+)-儿茶素-4-羟基聚糖酶和亮蓝蛋白-4-脱氢酶均定位于KTC-1的胞浆中。这是首次报道好氧细菌通过亮蓝蛋白生物转化(+)-儿茶素为紫杉林。我们认为，热带泥炭可能成为降解天然芳香化合物的微生物的独特资源。(C)2007 Elsevier Inc.保留所有权利。
Peat contains various persistent compounds derived from plant materials. We isolated a novel (+)-catechin-degrading bacterium, Burkholderia sp. KTC-1 (KTC-1), as an example of a bacterium capable of degrading persistent aromatic compounds present in tropical peat. This bacterium was isolated by an enrichment technique and grew on (+)-catechin as the sole carbon source under acidic conditions. The reaction of a crude enzyme extract and a structural study of its products showed that (+)-catechin is biotransformed into taxifolin during the preliminary stages of its metabolism by KTC-1. HPLC analysis showed that this transformation occurs in two oxidation steps: 4-hydroxylation and dehydrogenation. Furthermore, both (+)-catechin 4-hydroxylanase and leucocyanidin 4-dehydrogenase were localized in the cytosol of KTC-1. This is the first report on biotransformation of (+)-catechin into taxifolin via leucocyanidin by an aerobic bacterium. We suggest that tropical peat could become a unique resource for microorganisms that degrade natural aromatic compounds. (C) 2007 Elsevier Inc. All rights reserved.