Function of the genetic element "Mona' associated with fungicide resistance in Monilinia fructicola
Function of the genetic element "Mona' associated with fungicide resistance in Monilinia fructicola
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蒙尼菌 (Monilinia fructicola) 基因元件“Mona”与杀菌剂抗性相关的功能
DOI：
10.1111/mpp.12387
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发表时间：
2017-01-01
影响因子：
4.9
通讯作者：
Luo, Chaoxi
中科院分区：
文献类型：
--
作者：
Chen, Shuning;Yuan, Nannan;Luo, Chaoxi
先前已证明遗传元件 Mona' 与 Monilinia fructicola 的去甲基化抑制剂 (DMIs) 抗性有关。在这项研究中，Mona'元件的启动子活性得到了遗传证明，并通过一系列缺失将活性范围缩小到20 bp的活性区域。 Mona' 敲除转化体 (Mona) 由 DMI 抗性分离株 Bmpc7 产生，与亲本分离株相比，转化体中的 EC50 值和 MfCYP51 基因的表达被发现降低。当Mona'元件插入DMI敏感分离株HG3的MfCYP51基因上游区域时，与亲本敏感分离株相比，转化子中MfCYP51基因的EC50值和表达量增加。这些结果表明，Mona'元件通过上调下游MfCYP51基因的表达来决定DMI杀菌剂抗性。在敲除和插入转化体中没有观察到适应性损失，即转化体与其亲本分离株相比表现出相似的菌丝生长速率、孢子形成和引起果实病变的能力，这表明Mona'元件不影响基础生命活动。
The genetic element Mona' has been shown previously to be associated with resistance to demethylation inhibitors (DMIs) in Monilinia fructicola. In this study, the promoter activity of the Mona' element was demonstrated genetically and the activity was narrowed down to a 20-bp active region through a series of deletions. Mona' knockout transformants (Mona) were generated from DMI-resistant isolate Bmpc7, and EC50 values and expression of the MfCYP51 gene were found to be reduced in transformants compared with the parental isolate. When the Mona' element was inserted into the upstream region of the MfCYP51 gene of the DMI-sensitive isolate HG3, the EC50 values and expression of the MfCYP51 gene increased in the transformants compared with the parental sensitive isolate. These results indicate that the Mona' element determines the DMI fungicide resistance through the up-regulation of the expression of the downstream MfCYP51 gene. No fitness penalty was observed in knockout and insertion transformants, i.e. transformants showed similar mycelial growth rate, sporulation and ability to cause lesions on fruit compared with their parental isolates, suggesting that the Mona' element does not affect basal life activities.