Adenosine-3',5'-monophosphate-dependent protein kinase from bovine anterior pituitary gland. 3. Structural specificity of the ATP site of the catalytic subunit.
Adenosine-3',5'-monophosphate-dependent protein kinase from bovine anterior pituitary gland. 3. Structural specificity of the ATP site of the catalytic subunit.
复制标题
来自牛垂体前叶的腺苷-3,5-单磷酸依赖性蛋白激酶。
DOI：
10.1139/o74-021
复制
发表时间：
1974
期刊：
影响因子：
--
通讯作者：
M. Gauthier
中科院分区：
文献类型：
--
作者：
S. Lemaire;F. Labrie;M. Gauthier
使用纯化的腺垂体蛋白激酶催化亚基测量了 3',5'-单磷酸腺苷（环 AMP）和 ATP 的类似物和衍生物对 [γ-32P]ATP 中的 32P 掺入组蛋白的影响。 γ 标记的 CTP、GTP 和 UTP 不能替代 [γ-32P]ATP，但当它们以未标记的化合物形式存在时，会轻微抑制 [γ-32P]-ATP 的磷酸化。观察到腺嘌呤核对 ATP 位点竞争能力的严格要求，腺嘌呤、腺苷、5'AMP 和 ADP 分别观察到 42%、39%、32% 和 63% 的抑制，而相应的嘌呤或嘧啶衍生物在相对于 [γ-32P]ATP 摩尔过量 13 倍时没有效果。环AMP的N6-苯甲酰基和N6-丁酰基衍生物是无活性的，而8-取代的衍生物通常与环AMP本身一样具有活性，但8-氨基和8-羟基衍生物除外，它们表现出较低的竞争程度。所有环状 AMP 和 A...
The effect of analogues and derivatives of adenosine 3′,5′-monophosphate (cyclic AMP) and of ATP on the incorporation of 32P from [γ-32P]ATP into histones has been measured using the purified catalytic subunit of adenohypophyseal protein kinase. Gamma-labeled CTP, GTP, and UTP cannot substitute for [γ-32P]ATP but they slightly inhibit the phosphorylation by [γ-32P]-ATP when present as unlabeled compounds. A stringent requirement of the adenine nucleus is observed for the ability to compete at the ATP site, inhibitions of 42, 39, 32, and 63% being observed respectively with adenine, adenosine, 5′AMP, and ADP, while the corresponding purine or pyrimidine derivatives have no effect when present at a 13-fold molar excess relative to [γ-32P]ATP. The N6-benzoyl and N6-butyryl derivatives of cyclic AMP are inactive whereas the 8-substituted derivatives are generally as active as cyclic AMP itself, except for the 8-amino- and 8-hydroxy-derivatives, which exhibit a lower degree of competition. All cyclic AMP and A...