A Drosophila PIAS homologue negatively regulates stat92E
A Drosophila PIAS homologue negatively regulates stat92E
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DOI：
10.1073/pnas.171302098
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发表时间：
2001-08-14
影响因子：
11.1
通讯作者：
Darnell, JE
中科院分区：
文献类型：
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作者：
Betz, A;Lampen, N;Darnell, JE
通过激活酪氨酸磷酸化的STATs（信号转导和转录激活因子）的转录激活，因此其生理影响可能会受到称为PIAS（激活STATs的蛋白质抑制剂）的蛋白质的负调控，正如之前在培养的哺乳动物细胞中进行的实验所显示的那样。本研究通过对果蝇进行基因修饰，证明了果蝇同源基因stat92E与果蝇PIAS基因（dpias）在体内的功能相互作用。为此，我们使用LOF等位基因，并在JAK-STAT途径突变背景下有条件地过表达dpias。我们得出结论，正确的dpias/stat92E比例对血细胞和眼睛发育至关重要。
Transcriptional activation by, and therefore the physiologic impact of, activated tyrosine-phosphorylated STATs (signal transducers and activators of transcription) may be negatively regulated by proteins termed PIAS (protein inhibitors of activated stats), as shown by previous experiments with mammalian cells in culture. Here, by using the genetic modifications in Drosophila, we demonstrate the in vivo functional interaction of the Drosophila homologues stat92E and a Drosophila PIAS gene (dpias). To this end we use a LOF allele and conditionally overexpressed dpias in JAK-STAT pathway mutant backgrounds. We conclude that the correct dpias/stat92E ratio is crucial for blood cell and eye development.