Endothelial cell activation following moderate traumatic brain injury
Endothelial cell activation following moderate traumatic brain injury
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DOI：
10.1179/016164101101198514
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发表时间：
2001-03-01
影响因子：
1.9
通讯作者：
Dore-Duffy, P
中科院分区：
文献类型：
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作者：
Balabanov, R;Goldman, H;Dore-Duffy, P
创伤性脑损伤可引起一连串的急性和慢性损伤反应，包括脑血管系统的紊乱，可能导致微血管内皮细胞的激活和功能障碍的内皮细胞发育。本研究在中度颅脑损伤的撞击模型中检测血管内皮细胞(EC)的激活。在这些研究中使用的内皮激活的标准是一些标记的表面表达，统称为内皮激活抗原。E-选择素(E-选择素)、血管细胞黏附分子(VACM-1)(CD106)、细胞间黏附分子-1(ICAM-1)(CD54)、葡萄糖转运蛋白(GLUT-1)、转铁蛋白受体(CD71)和MHC-I类分子的表达在冲击诱导后不同时间发生变化，并持续至冲击后2 h(PI)。免疫反应性GLUT-1和TfR的表达在PI后2~4h开始下降，并在PI后24 h保持较低水平。未见VCAM-1、MHC-II类分子或MHC-I类分子表达改变。EC激活的变化主要在撞击部位观察到，并暂时减弱。这些结果表明，轻度脑震荡损伤会导致内皮细胞的激活。
Traumatic brain injury (TBI) initiates a cascade of acute and chronic injury responses which include disturbances in the cerebrovasculature that may result in the activation of the microvascular endothelial development of a dysfunction endothelium. The present study examines endothelial cell (EC) activation in a percussion model of moderate TBI. The criteria for endothelial activation used in these studies was surface expression of a number of markers collectively termed endothelial activation antigens. Temporal induction of the major histocompatibility (MHC) class II molecules, E-selectin (CD62E), vascular cell adhesion molecule (VACM-1) (CD106) as well as altered expression of constitutively expressed intercellular adhesion molecule-1 (ICAM-1) (CD54), the glucose transporter protein (glut-1), the transferrin receptor (tfR) (CD71), and MHC class I molecules was examined at various times following impact Induction of E-selectin and increased expression of ICAM-1 was seen by 2 h post-impact (PI) and was sustained through 24 h PI. Decreased expression of immunologically reactive glut-1 and tfR was observed by 2-4 h PI and remained low up to 24 h PI. No induction of VCAM-1, MHC class II molecules or altered constitutive expression or MHC class I molecules was seen. Changes in EC activation were observed predominantly at the site of impact and were diminished temporarily. These results indicate that mild concussive injury to the brain results in activation of the endothelium.