LSPR Sensing of Molecular Biothiols Based on Noncoupled Gold Nanorods
LSPR Sensing of Molecular Biothiols Based on Noncoupled Gold Nanorods
复制标题
基于非耦合金纳米棒的分子生物硫醇的局域表面等离子体共振传感
DOI：
10.1021/la101285r
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发表时间：
2010-06-01
期刊：
影响因子：
3.9
通讯作者：
Zhao, Yibing
中科院分区：
文献类型：
--
作者：
Li, Chong;Wu, Chuanliu;Zhao, Yibing
经 mPEG-SH 分子保护的 Au NR（mPEG-Au NR）被证明是基于 LSPR 传感水溶液中分子生物硫醇的有前途的平台。表面mPEG-SH分子赋予Au NRs良好的稳定性和生物相容性，并且不会对生物大分子进行非特异性吸附。 mPEG-Au NR 的 LSPR 谱带在光谱偏移方面表现出稳定性和线性响应：其周围折射率的变化，灵敏度为 252 nm/RIU。 Au NR 周围的 mPEG-SH 松散结构提供了自由位点，从而允许分子生物硫醇结合到 Au NR 的表面。然后研究了 Au NR 对生物硫醇（如 GSH、Cys、Hcy、TGA、GSSG 和 BSA）的 LSPR 响应和敏感性。
Au NRs protected with mPEG-SH molecules (mPEG-Au NRs) were demonstrated to be a promising platform for LSPR-based sensing of molecular biothiols in aqueous solution. Surface mPEG-SH molecules endow Au NRs with great stability and biocompatibility and no nonspecific adsorption of biomacromolecules. The LSPR band of mPEG-Au NRs displays a stability and linear response in the spectral shift with respect to;: change in their surrounding refractive index with a sensitivity of 252 nm/RIU. The loose structure of mPEG-SH around the Au NRs offers free sites, thereby allowing molecular biothiols to bind onto the surfaces of Au NRs. The LSPR response and the sensitivity of Au NRs to biothiols such as GSH, Cys, Hcy, TGA, GSSG, and BSA were then studied.