In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe.
In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe.
复制标题
DOI：
10.1007/978-1-4939-7546-4_14
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
Yang J;Li F
染色质相关蛋白在基因表达、表观遗传调控、DNA修复、重组和复制等许多细胞过程中发挥着重要作用。特别是，表观遗传景观，由各种染色质结合蛋白，是动态的，并在上下文依赖的方式进行调节。染色质原位结合分析是研究蛋白质（如表观遗传组分）如何与染色质结合的有力而简单的工具。这种方法依赖于染色质结合蛋白质对去污剂提取更具抗性的事实。在这里，我们描述了一个协议，用于在裂殖酵母裂殖酵母原位染色质结合试验。
Chromatin-associated proteins play critical roles in many cellular processes, including gene expression, epigenetic regulation, DNA repair, recombination and replication. Especially, epigenetic landscape, shaped by a variety of chromatin-binding proteins, is dynamic and regulated in a context-dependent manner. In situ chromatin binding assay is a powerful but simple tool to investigate how proteins, such as epigenetic components, associate with chromatin. This approach relies on the fact that chromatin bound proteins are more resistant to detergent extraction. Here we describe a protocol for the in situ chromatin binding assay used in Schizosaccaromyces pombe.