Isolation and structural characterization of a pectin from Lycium ruthenicum Murr and its anti-pancreatic ductal adenocarcinoma cell activity
Isolation and structural characterization of a pectin from Lycium ruthenicum Murr and its anti-pancreatic ductal adenocarcinoma cell activity
复制标题
黑果枸杞果胶的分离、结构表征及其抗胰腺导管腺癌细胞活性
DOI：
10.1016/j.carbpol.2019.115104
复制
发表时间：
2019
影响因子：
11.2
通讯作者：
Kan Ding
中科院分区：
文献类型：
--
作者：
Shihai Zhang;Fei He;Xia Chen;Kan Ding
采用热水提取、乙醇沉淀的方法从黑果枸杞果实中获得粗多糖。采用DEAE Sepharose™Fast Flow 和Sephacryl S-300 HR 柱进行阴离子交换色谱纯化，得到相对分子量为114.8kDa 的均质多糖LRP3-S1。单糖组成分析表明，LRP3-S1由鼠李糖、半乳糖醛酸、半乳糖、木糖和阿拉伯糖组成，摩尔比为14.4:17.7:26.6:16.4:24.9。 LRP3-S1含有鼠李糖半乳糖醛酸I (RG-I)主链，其在鼠李糖单元的C-4处被侧链部分取代，其包括T-连接的β-D-Galp、1,3-连接的β-D-Galp、1,6-连接的β-D-Galp、1,3,6-连接的β-D-Galp、1,5-连接的α-L-Araf， 1,3,5-连接 α-L-Araf、T-连接 α-L-Araf 和 T-连接 β-D-Xylp。生物活性测试表明LRP3-S1能够抑制胰腺癌细胞的生长。此外，LRP3-S1可以减弱BxPC-3细胞的侵袭能力并下调p-FAK、p-AKT、p-GSK-3β和p-p38 MAP激酶的蛋白表达。
Crude polysaccharides were obtained from fruits ofLycium ruthenicumMurr using hot water extraction followed by ethanol precipitation. A homogeneous polysaccharide, LRP3-S1 with a relative molecular weight of 114.8 kDa was purified by anion-exchange chromatography on DEAE Sepharose™Fast Flow and Sephacryl S-300 HR column. Monosaccharide composition analysis revealed that LRP3-S1 was composed of rhamnose, galacturonic acid, galactose, xylose and arabinose in a molar ratio of 14.4: 17.7: 26.6: 16.4: 24.9. LRP3-S1 contained a rhamnogalacturonan I (RG-I) backbone partially substituted at C-4 of rhamnose units by side chains, which included T-linked β-D-Galp, 1,3-linked β-D-Galp, 1,6-linked β-D-Galp, 1,3,6-linked β-D-Galp, 1,5-linked α-L-Araf, 1,3,5-linked α-L-Araf, T-linked α-L-Arafand T-linked β-D-Xylp. Biological activity tests showed that LRP3-S1 could inhibit the growth of pancreatic cancer cells. In addition, LRP3-S1 could attenuate invasion ability of BxPC-3 cells and down-regulate protein expression of p-FAK, p-AKT, p-GSK-3β and p-p38 MAP kinase.