CHARACTERIZATION OF THE VASCULATURE WITHIN A MURINE ADENOCARCINOMA GROWING IN DIFFERENT SITES TO EVALUATE THE POTENTIAL OF VASCULAR THERAPIES
CHARACTERIZATION OF THE VASCULATURE WITHIN A MURINE ADENOCARCINOMA GROWING IN DIFFERENT SITES TO EVALUATE THE POTENTIAL OF VASCULAR THERAPIES
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DOI：
10.3109/02841869509093989
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发表时间：
1995-01-01
期刊：
影响因子：
3.1
通讯作者：
DOUBLE, JA
中科院分区：
文献类型：
--
作者：
COWEN, SE;BIBBY, MC;DOUBLE, JA
许多花瓶活性剂可以影响皮下生长的实验性实体瘤的脉管系统，但这些模型不太可能反映人类转移性疾病的脉管系统。本研究描述了一种转移至肝脏的小鼠原位结肠肿瘤。盲肠肿瘤的形态和血管模式与皮下肿瘤相似。肿瘤。盲肠肿瘤静脉注射去甲肾上腺素（NA）（160μgkg(-1)）和肼屈嗪（HDZ）（10mgkg(-1)）引起的血管闭塞率分别为32%和59%，而皮下肿瘤的血管闭塞率分别为35%和78%。肿瘤。肝转移瘤和静脉注射产生的全身沉积物之间存在显着的形态学差异。肿瘤细胞的接种。原位移植后的肝转移含有功能性脉管系统，但 NA 或 HDZ 未观察到明显的闭塞。原位植入引起的继发性疾病的血管发育和形态学外观表明，这将是研究通过血管或抗血管生成机制起作用的药物的有用模型。
Numerous vase-active agents can affect vasculature in experimental solid tumours growing subcutaneously (s.c.), but these models are unlikely to reflect the vasculature of metastatic disease in man. The present study describes a murine orthotopic colon tumour which metastasises to the liver. Morphology and vascular pattern of caecal tumours is similar to s.c. tumours. Vascular occlusion caused by intravenous (i.v.) noradrenaline (NA) (160 mu gkg(-1)) and hydralazine (HDZ) (10 mgkg(-1)) was 32% and 59% respectively for the caecal tumours compared with 35% and 78% for s.c. tumours. Significant morphological differences were seen between liver metastases and systemic deposits produced by i.v. inoculation of tumour cells. Liver metastases following orthotopic transplantation contained functional vasculature but no significant occlusion was seen with NA or HDZ. The vascular development and morphological appearance of secondary disease resulting from orthotopic implantation suggests that this would be a useful model for the study of agents that act either by vascular or anti-angiogenic mechanism.