Prolactin inhibits annexin 5 expression and apoptosis in the corpus luteum of pseudopregnant rats: Involvement of local gonadotropin-releasing hormone
Prolactin inhibits annexin 5 expression and apoptosis in the corpus luteum of pseudopregnant rats: Involvement of local gonadotropin-releasing hormone
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DOI：
10.1210/en.2003-0118
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发表时间：
2003-08-01
期刊：
影响因子：
4.8
通讯作者：
Hashimoto, I
中科院分区：
文献类型：
--
作者：
Kawaminami, M;Shibata, Y;Hashimoto, I
我们研究了膜联蛋白5和催乳素在假孕大鼠黄体中的表达之间的特定关系，特别关注GnRH和黄体细胞凋亡。卵巢膜联蛋白5 mRNA的表达在假孕中期显著下降，并在结束时恢复，而在妊娠的相应日期仍然很低。多巴胺受体激动剂CB-154，在中期pseudopregulatory（d 5），增加卵巢膜联蛋白5 mRNA，而催乳素，每天给3天的周期大鼠，减少it.A免疫细胞化学研究还表明，膜联蛋白5增加黄体d 6和7 pseudopregulatory后，CB-154治疗d 5。膜联蛋白5阳性细胞在黄体中的分布不均匀，与末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记（TUNEL）阳性细胞相匹配。由于GnRH刺激促性腺激素细胞膜联蛋白5 mRNA表达，因此检查了GnRH受体的参与。同时接受CB-154的假孕大鼠的单侧卵巢囊局部给予GnRH拮抗剂Cetrorelix，废除了膜联蛋白5的表达和TUNEL反应。目前的结果清楚地表明，催乳素减少膜联蛋白5的mRNA在黄体细胞在假孕。催乳素被认为抑制GnRH的局部作用，GnRH刺激膜联蛋白5的合成和假孕期间功能性黄体细胞的凋亡。
We investigated a specific relationship between the expression of annexin 5 and prolactin in the corpus luteum of pseudopregnant rats, with particular interest in GnRH and apoptosis of luteal cells. The expression of ovarian annexin 5 mRNA was significantly decreased at mid-pseudopregnancy and recovered at the end, whereas it remained low on the corresponding day of pregnancy. The dopamine agonist CB-154, administered at mid-pseudopregnancy (d 5), increased ovarian annexin 5 mRNA, whereas prolactin, given daily for 3 d to cycling rats, decreased it. An immunocytochemical study also showed that annexin 5 increased in the corpus luteum on d 6 and 7 of pseudopregnancy after treatment with CB-154 on d 5. The distribution of annexin 5-positive cells was not uniform in the corpus luteum and matched that of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL)-positive cells. Because GnRH stimulates annexin 5 mRNA expression in the gonadotropes, involvement of the GnRH receptor was examined. Local administration of a GnRH antagonist, Cetrorelix, to hemilateral ovarian bursa of pseudopregnant rats simultaneously receiving CB-154 abrogated both the expression of annexin 5 and the TUNEL reaction. The present results clearly demonstrate that prolactin decreases annexin 5 mRNA in the luteal cells during pseudopregnancy. Prolactin is suggested to suppress the local action of GnRH, which stimulates annexin 5 synthesis and apoptosis of functional luteal cells during pseudopregnancy.