Three-photon excited fluorescence imaging of unstained tissue using a GRIN lens endoscope.
Three-photon excited fluorescence imaging of unstained tissue using a GRIN lens endoscope.
复制标题
DOI：
10.1364/boe.4.000652
复制
发表时间：
2013-05-01
影响因子：
3.4
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Huland DM;Charan K;Ouzounov DG;Jones JS;Nishimura N;Xu C
我们提出了一种紧凑且便携的三光子梯度折射率（GRIN）透镜内窥镜系统，该系统适用于对未染色组织进行成像，有可能对身体深处进行成像，它使用直径为1毫米、长度为8厘米的GRIN透镜系统。在水中的横向分辨率和轴向分辨率分别为1.0微米和9.5微米。使用基于光纤的1040纳米激发源，以每秒2帧的速度对直径约200微米的视野进行成像。在平均功率为5.9毫瓦的情况下，对未染色的小鼠肺进行了离体成像演示。这些结果证明了三光子GRIN透镜内窥镜用于光学活检的可行性。
We present a compact and portable three-photon gradient index (GRIN) lens endoscope system suitable for imaging of unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The lateral and axial resolution in water is 1.0 μm and 9.5 μm, respectively. The ~200 μm diameter field of view is imaged at 2 frames/s using a fiber-based excitation source at 1040 nm. Ex vivo imaging is demonstrated with unstained mouse lung at 5.9 mW average power. These results demonstrate the feasibility of three-photon GRIN lens endoscopy for optical biopsy.