Listeria monocytogenes in Smoked Salmon and Other Smoked Fish at Retail in Italy: Frequency of Contamination and Strain Characterization in Products from Different Manufacturers
Listeria monocytogenes in Smoked Salmon and Other Smoked Fish at Retail in Italy: Frequency of Contamination and Strain Characterization in Products from Different Manufacturers
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DOI：
10.4315/0362-028x.jfp-15-599
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发表时间：
2017-02-01
影响因子：
2
通讯作者：
Migliorati, Giacomo
中科院分区：
文献类型：
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作者：
Acciari, Vicdalia Aniela;Torresi, Marina;Migliorati, Giacomo
2011年5月至12月期间在意大利市场购买了来自12个欧盟国家50家不同制造商的778个包装熏鱼样本（774个熏鱼和4个熏鱼）。在售样品的表面温度介乎0至130摄氏度（3.4 +/- 1.5℃，平均±标准差）。778份样品中有680份（87.4%）以100 CFU/g保存。最大污染水平为1.3 × 10(6) CFU/g。国家（chi(2) = 91.54, P < 0.05）和生产商（chi(2) = 193.22, P < 0.05）之间的熏鱼污染水平差异显著。不同生产场所产品的检测频率从0到76.9%不等。血清学凝集分型显示，检测到的主要血清型为1/2a（65.3%）和1/2b（22.4%）。用限制性内切酶AscI和ApaI进行脉冲场凝胶电泳分型，从144株分离物中获得36个脉冲型，聚类为17个类群。8种主要脉冲型占分离株的70.8%。其中三种主要脉冲型仅来自同一制造商的产品。一般来说，来自同一制造商的产品表现出遗传同质性，具有一种强烈流行的脉冲型。不同的生产厂家通常表现出非常不同的污染程度的最终产品，确认过程卫生管理的重要性，以控制单核增生乳杆菌污染。
Seven hundred seventy-eight samples of packaged smoked fish (774 smoked salmon and 4 smoked swordfish) on sale in Italy, from 50 different manufacturers located in 12 European Union countries, were purchased from the Italian market between May and December 2011. The surface temperatures of the samples on sale ranged from 0 to 130 degrees C (3.4 +/- 1.5 degrees C, mean +/- SD). Six hundred eighty (87.4%) of 778 samples were stored at 100 CFU/g. The maximum level of contamination was 1.3 X 10(6) CFU/g. The differences in the level of contamination of smoked fish between countries (chi(2) = 91.54, P < 0.05) and manufacturers (chi(2) = 193.22, P < 0.05) were significant. The frequency of detection for products from different manufacturing premises ranged from 0 to 76.9%. Serotyping by serological agglutination revealed that the main serotypes detected were 1/2a (65.3%) and 1/2b (22.4%). Pulsed-field gel electrophoresis typing with restriction enzymes AscI and ApaI yielded 36 pulsotypes from 144 isolates, clustering into 17 groups. Eight main pulsotypes accounted for 70.8% of the isolates. Three of the main pulsotypes were exclusively from products of a single manufacturer. In general, products from the same manufacturer showed genetic homogeneity, with one strongly prevalent pulsotype. Different manufacturers usually showed very different levels of contamination of the final product, confirming the importance of the management of process hygiene for controlling L. monocytogenes contamination.