Dietary supplementation of glycine modulates inflammatory response indicators in broiler chickens.
Dietary supplementation of glycine modulates inflammatory response indicators in broiler chickens.
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日粮中添加甘氨酸可调节肉鸡的炎症反应指标。
DOI：
10.1017/s0007114508966125
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Y. Akiba
中科院分区：
文献类型：
--
作者：
Kazuaki Takahashi;Akira Aoki;T. Takimoto;Y. Akiba
进行了三个实验来研究日粮中添加甘氨酸 (Gly) 对以玉米和豆粕为主要成分的基础日粮喂养的肉鸡炎症反应的影响。通过分析实验 1 和 2 中硝酸盐加亚硝酸盐 (NOx) 和铜蓝蛋白 (Cer) 的血浆浓度，检查脂多糖 (LPS) 注射后的炎症相关过程，或检查脾脏和肝脏中多个基因的表达，包括 IL-1 beta 和 -6、TNF 样配体 (TL)1A、诱导型 NO 合酶、干扰素 (IFN)-gamma 和 Toll 样受体 (TLR) 4实验3中，还测定了实验2中通过LPS和Sephadex注射进行免疫刺激后的生长性能。在实验1中，饲喂补充有10或20g/kg甘氨酸的饮食的鸡比饲喂补充有0或40g/kg甘氨酸的饮食的鸡表现出较低的血浆NOx和Cer反应。在实验 2 中，当雏鸡饲喂含甘氨酸或谷氨酸 (Glu) 的等氮日粮时，添加 10 g/kg 甘氨酸对血浆 NOx 和 Cer 也有类似的影响。补充 Gly 的饮食喂养的鸡表现出比补充 Glu 的饮食喂养的鸡更好的生长性能。饲喂添加 10 g/kg 日粮的甘氨酸的鸡的脾脏表达炎症反应相关基因低于实验 3 中饲喂基础日粮的鸡。这些结果表明，日粮添加甘氨酸部分通过改变促炎细胞因子（如 IL-1、IL-6、IFN-gamma 和 TL1A）的表达来调节炎症反应。
Three experiments were conducted to investigate the effect of dietary glycine (Gly) supplementation on inflammatory responses in broiler chicks fed a basal diet using maize and soybean meal as the primary ingredients. Inflammation-related processes following lipopolysaccharide (LPS) injection were examined by analysing plasma concentrations of nitrate plus nitrite (NOx) and ceruloplasmin (Cer) in experiments 1 and 2, or expression of several genes in the spleen and liver including IL-1 beta and -6, TNF-like ligand (TL)1A, inducible NO synthase, interferon (IFN)-gamma and toll-like receptor (TLR) 4 were examined in experiment 3. Growth performance was also determined following immunological stimulation by both LPS and Sephadex injection in experiment 2. In experiment 1, birds fed a diet supplemented with Gly at 10 or 20 g/kg showed lower responses in plasma NOx and Cer than birds fed the diet supplemented with Gly at 0 or 40 g/kg. In experiment 2, a similar effect of Gly supplementation at 10 g/kg on plasma NOx and Cer was observed when chicks were fed either an isonitrogenous diet with Gly or glutamic acid (Glu). Gly-supplemented diet-fed birds showed better growth performance than Glu-supplemented diet-fed birds. The splenic expression of inflammatory response-related genes in birds fed a diet supplemented with Gly at 10 g/kg diet was lower than that of birds fed the basal diet in experiment 3. These results suggest that dietary Gly supplementation modulates the inflammatory response partly through changes in the expression of pro-inflammatory cytokines such as IL-1, IL-6, IFN-gamma and TL1A.
DOI：
10.1152/ajpgi.1996.271.1.g97
发表时间：
1996-07-01
影响因子：
4.5
作者：
Ikejima, K;Iimuro, Y;Thurman, RG
通讯作者：
Thurman, RG