Template-directed conjugation of heterogeneous oligonucleotides to a homobifunctional molecule for programmable supramolecular assembly.
Template-directed conjugation of heterogeneous oligonucleotides to a homobifunctional molecule for programmable supramolecular assembly.
复制标题
异源寡核苷酸与同双功能分子的模板定向缀合，用于可编程超分子组装。
作者：
Helmi S
纳米科学渴望模仿自然对功能分子组合的控制。在这里，我们提出了一种模板技术，用于将两种不同的寡核苷酸有效地附着到一个同源双功能分子上，使其能够在DNA纳米结构中控制和可编程的放置。我们展示了它在一系列具有不同共轭化学和水溶性的有机分子中的应用。我们发现，这两个寡核苷酸适配器可以用来将双功能菁染料整合到自组装的三维DNA折纸纳米结构中，从而控制位置和方向。我们还演示了使用这两个适配器通过一系列链位移反应对目标分子的环境施加动态控制。
Nanoscience aspires to mimic nature's control over functional molecular assemblies. Here we present a templating technique for the efficient attachment of two different oligonucleotides to a homobifunctional molecule, enabling its controlled and programmable placement within a DNA nanostructure. We demonstrate its application to a range of organic molecules with different conjugation chemistries and water solubilities. We show that the two oligonucleotide adapters can be used to integrate a bifunctional cyanine dye into a self-assembled three-dimensional DNA origami nanostructure, giving control of both position and orientation. We also demonstrate the use of both adapters to exert dynamic control over the environment of the target molecule by means of a series of strand-displacement reactions.