Binding of 9-aminoacridine to deoxydinucleoside phosphates of defined sequence: preferences and stereochemistry.
Binding of 9-aminoacridine to deoxydinucleoside phosphates of defined sequence: preferences and stereochemistry.
复制标题
9-氨基吖啶与确定序列的脱氧二核苷磷酸的结合：偏好和立体化学。
DOI：
10.1016/0022-2836(81)90315-6
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发表时间：
1981
影响因子：
5.6
通讯作者：
Kallenbach,NR
中科院分区：
文献类型：
--
作者：
Young,PR;Kallenbach,NR
用质子核磁共振研究了9-氨基吖啶与不同序列的脱氧二核苷磷酸的相互作用，研究了序列对9-氨基吖啶与DNA结合的影响。定量结合信息可以通过比较质子化学位移行为的9-氨基吖啶后，加入磷酸二核苷的各种模型的相互作用，使用最小二乘计算机拟合程序。拟合数据的最简单模型包括（1）9-氨基吖啶的二聚化和（2）1：1和2：1（磷酸二核苷/9-氨基吖啶）复合物的混合物。计算的参数允许不同序列的结合常数和立体化学的比较。1：1复合物似乎涉及环氮与骨架磷酸盐的相互作用和吖啶上一个或两个发色团的堆叠;观察到交替（嘌呤-嘧啶或嘧啶-嘌呤）的结合优先于非交替（嘌呤-嘌呤）二核苷磷酸盐。2：1复合物涉及吖啶在两个互补的二核苷磷酸链之间的嵌入，在结合中具有弱序列偏好。非交替嘌呤-嘌呤序列和交替嘧啶-嘌呤或嘌呤-嘧啶序列之间的嵌入立体化学的不同之处在于9-氨基吖啶与一条链的嘌呤堆叠而不是跨越相对链上的嘌呤。立体化学的差异可能是移码序列特异性的决定因素。
The effect of sequence on the binding of 9-aminoacridine to DNA has been investigated by studying its interaction with deoxydinucleoside phosphates of different sequences using proton nuclear magnetic resonance. Quantitative binding information can be obtained by comparison of the proton chemical shift behavior of 9-aminoacridine upon addition of dinucleoside phosphate to various models for the interaction using least-squares computer fitting procedures. The simplest model that fits the data includes (1) dimerization of 9-aminoacridine and (2) a mixture of 1:1 and 2:1 (dinucleoside phosphate/9-aminoacridine) complexes. The computed parameters allow comparison of binding constants and stereochemistry for different sequences. The 1:1 complexes seem to involve interaction of the ring nitrogen with the backbone phosphate and stacking of one or both chromophores on the acridine; preference in binding is observed for alternating (purine-pyrimidine or pyrimidine-purine) over non-alternating (purine-purine) dinucleoside phosphates. The 2:1 complexes involve intercalation of the acridine between two complementary dinucleoside phosphate strands with weak sequence preferences in binding. The stereochemistry of intercalation differs between non-alternating purine-purine sequences and the alternating pyrimidine-purine or purine-pyrimidine sequences in having the 9-aminoacridine stacked with the purines of one strand rather than straddling the purines on opposite strands. The difference in stereochemistry could possibly be a determining factor in frameshift sequence specificity.