Structural and serological studies on a new 4-deoxy-D-arabino-hexose-containing O-specific polysaccharide from the lipopolysaccharide of Citrobacter braakii PCM 1531 (serogroup O6)
Structural and serological studies on a new 4-deoxy-D-arabino-hexose-containing O-specific polysaccharide from the lipopolysaccharide of Citrobacter braakii PCM 1531 (serogroup O6)
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DOI：
10.1046/j.1432-1033.2003.03640.x
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发表时间：
2003-07-01
期刊：
影响因子：
--
通讯作者：
Knirel, YA
中科院分区：
文献类型：
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作者：
Katzenellenbogen, E;Kocharova, NA;Knirel, YA
通过脂多糖（LPS）的温和酸水解分离出Citrobacter braakii PCM 1531（血清群O 6）的O-特异性多糖，发现其含有摩尔比为2：1：1：1的d-岩藻糖、l-鼠李糖、4-脱氧-d-阿拉伯糖-己糖和O-乙酰基。基于甲基化分析和（1）H和（13）C NMR光谱数据，确定了O-特异性多糖的支链四糖重复单元的结构。使用各种血清学测定，证明菌株PCM 1531的LPS与来自柠檬酸杆菌PCM 1487（血清群O 5）或C.大肠杆菌PCM 1488（血清群O36）。柠檬酸杆菌属的另外两种菌株PCM 1504和PCM 1505与菌株PCM 1531一起被归类为血清群O 6，但在血清学上与菌株PCM 1531不同，应重新归类为另一血清群。
The O-specific polysaccharide of Citrobacter braakii PCM 1531 (serogroup O6) was isolated by mild acid hydrolysis of the lipopolysaccharide (LPS) and found to contain d-fucose, l-rhamnose, 4-deoxy-d-arabino -hexose and O-acetyl groups in molar ratios 2 : 1 : 1 : 1. On the basis of methylation analysis and (1) H and (13) C NMR spectroscopy data, the structure of the branched tetrasaccharide repeating unit of the O-specific polysaccharide was established. Using various serological assays, it was demonstrated that the LPS of strain PCM 1531 is not related serologically to other known 4-deoxy-d-arabino -hexose-containing LPS from Citrobacter PCM 1487 (serogroup O5) or C. youngae PCM 1488 (serogroup O36). Two other strains of Citrobacter , PCM 1504 and PCM 1505, which, together with strain PCM 1531, have been classified in serogroup O6, were shown to be serologically distinct from strain PCM 1531 and should be reclassified into another serogroup.