Inhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B
Inhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B
复制标题
细胞松弛素 B 去核细胞中 B 组虫媒病毒抗原产生和复制的抑制
作者：
K. Kos;B. Osborne;R. Goldsby
对辛德毕斯（SIN）病毒（A组虫媒病毒（披膜病毒））和日本脑炎（JE）病毒（代表性的B组虫媒病毒（披膜病毒））在去核和有核细胞中的生长进行了比较研究。免疫荧光试验和产量测定表明，去核后感染SIN病毒的鸡胚细胞产生病毒特异性抗原和感染性病毒。相比之下，乙脑未能复制或产生病毒特异性抗原的细胞已被去核前，甚至2小时后感染。在感染后不同时间的enulceation的影响的研究表明，一个核必须存在至少2小时，可能长达4小时后，感染产生乙脑特异性抗原或传染性乙脑病毒。这些研究表明，SIN，A组虫媒病毒（披膜病毒），它没有核的要求，形成鲜明对比的B组虫媒病毒（披膜病毒），乙脑，这可能有一个最初的依赖于核相关的过程。
A comparative study of the growth of Sindbis (SIN) virus, a group A arbovirus (togavirus), and Japanese encephalitis (JE) virus, a representative group B arbovirus (togavirus), was conducted in enucleate and nucleate cells. Immunofluorescent tests and yield measurements demonstrated that chicken embryo cells which had been enucleated and subsequently infected with SIN virus produced virus-specific antigens and infectious virus. By contrast, JE failed to replicate or produce virus-specific antigen in cells which had been enucleated before or even 2 h post infection. Studies of the effect of enulceation at various times after infection demonstrated that a nucleus must be present at least 2 and possibly as long as 4 h after infection to produce either JE-specific antigen or infectious JE virus. These studies demonstrate that the replication of SIN, a group A arbovirus (togavirus), which has no nuclear requirement, contrasts sharply with that of a group B arbovirus (togavirus), JE, which may have an initial dependence on a nucleus-associated process.