Resistant keratinocytes in 7,12-dimethylbenz[a]anthracene-initiated hamster buccal pouch epithelium.
Resistant keratinocytes in 7,12-dimethylbenz[a]anthracene-initiated hamster buccal pouch epithelium.
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7,12-二甲基苯并[a]蒽引发的仓鼠颊囊上皮中的耐药角质形成细胞。
DOI：
10.1093/carcin/12.4.617
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发表时间：
1991
期刊：
影响因子：
4.7
通讯作者：
Solt,DB
中科院分区：
文献类型：
--
作者：
Hussong,JW;Polverini,PJ;Solt,DB
为了验证抗细胞毒性是癌前口腔黏膜上皮获得性的假设，我们从鼠颊袋上皮素（HBPE）、非启动型HBPE和恶性HIBPE细胞系中制备了细胞解离物。这些细胞类型对诱导致癌物7,12-二甲基苯[a]蒽（DMIBA）的细胞毒性作用进行了评估。利用有丝分裂抑制实验和克隆性实验来评估这些细胞在40 μM DMBA存在下复制或形成集落的能力。两种方法均能100%抑制原代镀HEPE细胞的复制。PO II是一种来源于未启动的石蜡油暴露HIBPE的细胞系，在有丝分裂抑制和集落形成实验中分别受到97%和100%的抑制。同样的细胞系，如原代镀IH{BPE，缺乏血管生成和不依赖于锚定生长的转化相关特性。相比之下，三种恶性HEPE细胞系，两种来自dmba启动的I-IIBPE长期培养，一种来自dm1ba诱导的HBPE癌，在细胞毒性抗性试验中仅被抑制34%或更少。从HBPE中获得的原代细胞培养物，每周两次局部应用0.5%的石蜡油DMBA溶液3或5周，在体内被抑制到中等程度，表明存在DMBA抗性细胞。此外，在完成5周初始方案后的2周、6周和10周制备的细胞培养物中观察到dmiba耐药细胞菌落。在这个实验模型中，耐药细胞的祖细胞在起始后在体内持续数周，可能代表早期肿瘤前细胞群。
In order to test the hypothesis that the property of resistance to cytotoxicity is an acquired trait of premalignant oral mucosal epithelium, cell dissociates were prepared fromin vivoinitiated hamster buccal pouch epitheliuin (HBPE), non-initiated HBPE and malignant HIBPE cell lines. These cell types were evaluated for resistance to the cytotoxic effects of the inducing carcinogen, 7,12-dimethylbenz[a]anthracene (DMIBA). A mitoinhibition assay and a clonogerncity assay were used to assess the ability of these cells to replicate or form colonies in the presence of 40 μM DMBA. Replication of primary plated HEPE cells was inhibited by 100% in both assays. PO II, a cell line derived from non-initiated, paraffin-oil-exposed HIBPE, was inhibited by 97 and 100% in the mitoinhibition and colony-forming assays respectively. This same cell line, like primary plated IH{BPE, lacked the transformation-linked traits of angiogenesis and anchorage-independent growth. By contrast, three malignant HEPE cell lines, two derived during long-term culture of DMBA-initiated I-IIBPE, and one from a DM1BA-induced HBPE cacinoma, were inhibited by only 34% or less in the assays for resistance to cytotoxicity. Primary cell cultures derived from HBPE initiatedin vivowith twice-weeldy topical applications of a 0.5% solution of DMBA in paraffin oil, for 3 or 5 weeks, were inhibited to an intermediate degree, indicating the presence of DMBA-resistant cells. In addition, DMIBA-resistant cell colonies were observed in cell cultures prepared at 2, 6 and 10 weeks after completing the 5 week initiation regimen. Progenitors of the resistant cells, persistingin vivofor several weeks after initiation, may represent early preneoplastic cell populations in this experimental model.