CHARACTERIZATION OF A GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN FROM BONE
CHARACTERIZATION OF A GAMMA-CARBOXYGLUTAMIC ACID-CONTAINING PROTEIN FROM BONE
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DOI：
10.1073/pnas.73.5.1447
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
RAMAN, N
中科院分区：
文献类型：
--
作者：
PRICE, PA;OTSUKA, AS;RAMAN, N
可以利用。从几种钙化组织中纯化出含-羧谷氨酸蛋白。3的存在。通过碱法水解和氨基酸分析，建立了牛蛋白中的-羧谷氨酸残基。-carboxyglutamic酸。的恒等式。通过质谱分析从碱性水解产物中分离的未知氨基酸，确定了牛蛋白中的-羧谷氨酸。该蛋白在EDTA脱矿过程中从细碎的骨中提取，并通过Sephadex G-100凝胶过滤和DEAE-Sephadex层析从EDTA提取物中纯化。该蛋白的分子量为6800 MW，等电点pH值为3.7。确定了氨基末端的15个残基，并确定该蛋白不是. γ的片段。-含羧谷氨酸的凝血因子。类似的可以。含-羧谷氨酸的蛋白也从牛牙本质、剑鱼脊椎骨和人胫骨中纯化出来。不可以。-羧谷氨酸可在弹力支的钙化软骨、小牛骨骺生长软骨或牛牙釉质中检测到。牛蛋白与羟基磷灰石结合强烈，但不与无定形磷酸钙结合，它是羟基磷灰石从过饱和钙和磷酸盐溶液中结晶的有效抑制剂。
A .gamma.-carboxyglutamic acid-containing protein was purified from several calcified tissues. The presence of 3 .gamma.-carboxyglutamic acid residues in the bovine protein was established by alkaline hydrolysis and amino acid analysis, a method based upon studies with synthetic .gamma.-carboxyglutamic acid. The identity of .gamma.-carboxyglutamic acid in the bovine protein was established by mass spectroscopy on the unknown amino acid isolated from alkaline hydrolysates. The protein is extracted from finely ground bone during demineralization with EDTA, and purified from EDTA extracts by gel filtration over Sephadex G-100 and chromatography on DEAE-Sephadex. The protein has a 6800 MW and an isoelectric point of pH 3.7. The amino-terminal 15 residues were determined, and establish that this protein is not a fragment of the .gamma.-carboxyglutamic acid-containing blood clotting factors. Similar .gamma.-carboxyglutamic acid-containing proteins also were purified from bovine dentine, swordfish vertebrae, and human tibia. No .gamma.-carboxyglutamic acid can be detected in the calcified cartilage of elasmobranches, in calf epiphyseal growth cartilage, or in bovine tooth enamel. The bovine protein binds strongly to hydroxyapatite but not to amorphous calcium phosphate, and it is a potent inhibitor of hydroxyapatite crystallization from supersaturated solutions of Ca and phosphate.