Microtomographic Analysis of Neuronal Circuits of Human Brain
Microtomographic Analysis of Neuronal Circuits of Human Brain
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DOI：
10.1093/cercor/bhp237
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发表时间：
2010-07-01
期刊：
影响因子：
3.7
通讯作者：
Suzuki, Yoshio
中科院分区：
文献类型：
--
作者：
Mizutani, Ryuta;Takeuchi, Akihisa;Suzuki, Yoshio
我们报告了人类大脑皮层神经元回路的 3D 分析。大脑功能所必需的神经元回路是由神经元构建成的 3D 网络，因此追踪人类大脑皮层的 3D 神经元网络是了解人类大脑功能机制的第一步。通过金属浸渍染色的成人额叶皮层组织的 X 射线显微断层扫描成像来可视化皮层微观结构。通过追踪 X 射线吸收系数的 3D 分布来构建骨架线模型。获得的神经元模型由240个锥体神经元和131个中间神经元组成。还对毛细血管结构以及毛细血管腔中的血细胞进行可视化和追踪，以建立毛细血管网络模型。从骨架线模型中分析解决了可能的神经元回路。基于电路中的神经传递，讨论了已解析电路的运行机制。结果还表明，X 射线显微断层扫描是可视化大脑神经元回路的潜在方法。
We report a 3D analysis of the neuronal circuits of human cerebral cortex. Neuronal circuits, which are essential for brain functions, are built up by neurons as a 3D network, so tracing the 3D neuronal network of human cerebral cortex is the first step to understanding the mechanism of human brain functions. The cortical microstructures were visualized by X-ray microtomographic imaging of adult frontal cortex tissue stained with metal impregnation. Skeletonized wire models were built by tracing the 3D distribution of X-ray absorption coefficients. The obtained neuronal models were composed of 240 pyramidal neurons and 131 interneurons. Capillary vessel structures along with blood cells in the capillary lumen were also visualized and traced to build capillary network models. Possible neuronal circuits were analytically resolved from the skeletonized wire models. The operating mechanism of the resolved circuits is discussed on the basis of neurotransmission in the circuits. The results also indicate that X-ray microtomography is a potential method of visualizing the neuronal circuits of the brain.