Development of CryoVR, a virtual reality training system for hands-on cryoEM operations.
Development of CryoVR, a virtual reality training system for hands-on cryoEM operations.
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开发CryoVR，一种用于冷冻电镜实际操作的虚拟现实训练系统。
DOI：
10.1107/s2059798322005654
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发表时间：
2022-07-01
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本文描述了CryoVR的设计与评估，CryoVR是一种基于虚拟现实的冷冻电镜设备培训系统。CryoVR通过在安全且可及的虚拟环境中为用户提供冷冻电镜实验流程的体验，提供了独特的学习机会。
低温电子显微镜（cryoEM）已成为一种革命性的方法，用于解析高分辨率结构以及研究近天然状态下大分子复合物和病毒的动力学。然而，设备的可用性以及培训所需的时间和成本严重限制了培训机会。为解决这些问题，开发了一种基于虚拟现实的培训系统CryoVR，以便在操作实际的冷冻电镜设备之前对学员进行培训。本文描述了CryoVR（可在https://www.purdue.edu/cryoVR获取）的设计与评估，它帮助用户在虚拟环境中学习冷冻电镜实验流程，通过带有生动的视觉、音频和文字指导的逐步教程实现沉浸式培训。作为新手用户接触昂贵的实际冷冻电镜设备之前的一个培训步骤，CryoVR可通过多个培训模块帮助用户熟悉实际操作流程，并在通过内置的考试模式后获得证书。具有不同冷冻电镜经验水平的用户对CryoVR的定性评估和反馈表明，CryoVR作为一种全面的冷冻电镜流程培训工具具有重大价值。
This paper describes the design and assessment of CryoVR, a virtual reality-based training system for cryoEM devices. CryoVR provides a unique learning opportunity by giving users experience of cryoEM experimental procedures through a safe and accessible virtual environment. Cryogenic electron microscopy (cryoEM) has emerged as a revolutionary method for solving high-resolution structures and studying the dynamics of macromolecular complexes and viruses in near-native states. However, the availability of the equipment, and the time and cost needed for training, severely limit the opportunities for training. To solve these problems, a virtual reality-based training system, CryoVR, has been developed to prepare trainees before operating real-world cryoEM equipment. This paper describes the design and assessment of CryoVR (available at https://www.purdue.edu/cryoVR), which helps users learn cryoEM experimental procedures in a virtual environment, allowing immersive training with step-by-step tutorials with vivid visual, audio and text guidance. Implemented as a training step before a novice user interacts with the expensive real-world cryoEM equipment, CryoVR can help users to become familiar with hands-on operational procedures through multiple training modules and earning certificates after passing the built-in Exam mode. Qualitative evaluation and feedback of CryoVR from users with various levels of cryoEM experience indicate the substantial value of CryoVR as a tool for a comprehensive cryoEM procedural training.