Comparative study on physiological response characteristics of white clover to chloride salt and calcium-magnesium acetate (CMA) deicing agents under freeze-thaw stress
Comparative study on physiological response characteristics of white clover to chloride salt and calcium-magnesium acetate (CMA) deicing agents under freeze-thaw stress
复制标题
冻融胁迫下白三叶草对氯化盐和醋酸钙镁（CMA）除冰剂生理响应特性的比较研究
DOI：
10.1111/grs.12254
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发表时间：
2020
影响因子：
1.3
通讯作者：
Ding Xuemei
中科院分区：
文献类型：
--
作者：
Bian Wenjie;Bao Guozhang;Liao Jiafen;Zhang Qian;Chen Weiwei;Guan Shimei;Zhu Mengdan;Zhu Saning;Li Haiyi;Ding Xuemei
除冰剂的应用往往与冻融（FT）相吻合;然而，氯化钠除冰剂和浓缩醋酸钙镁（CMA）除冰剂在FT环境中对植物的生理效应尚未得到很好的研究。为探讨白色三叶草幼苗对不同除冰剂和FT胁迫的生理响应特性，以白色三叶草幼苗为材料，研究了FT和除冰剂（DA）模拟胁迫下幼苗可溶性总蛋白、可溶性糖、丙二醛（MDA）、超氧化物歧化酶（SOD）和过氧化物酶（POD）含量的变化。结果表明，FT胁迫下，白色三叶草幼苗可溶性蛋白质含量下降，MDA含量、可溶性糖含量、SOD和POD活性升高。在FT循环中，单一FT胁迫下的可溶性糖含量以及3个FT处理组的SOD和POD活性均与温度呈负相关。MDA与其他指标呈显著相关性，而上述其他生理指标之间相关性不显著（p< 0.05）。FT + DA复合胁迫可引起植物膜脂过氧化和渗透势失衡等伤害。作为一种环境友好型DA，浓缩CMA对植物的危害小于氯化钠DA，但仍不可避免地给植物带来危害。我们的研究结果和相应的讨论突出了两种防冻剂对FT胁迫下植物的影响的新发现。
The application of deicing agents often coincides with freeze–thaw (FT); however, the physiological effects of the sodium chloride deicing agent and concentrated calcium‐magnesium acetate (CMA) deicing agent on plants in the FT environment are not well investigated. To explore the physiological response characteristics of white clover seedlings under different kinds of deicing agents and FT stress, the changes in the content of total soluble protein, soluble sugar, malondialdehyde (MDA), superoxide dismutase (SOD) and peroxidase (POD) of white clover seedlings were determined under the simulated stress of FT and deicing agent (DA). The results indicated that the soluble protein content in the white clover seedlings decreased, whereas the contents of MDA and soluble sugar, and the activities of SOD and POD increased under the FT stress. In the FT cycle, the content of soluble sugar under single FT stress, and the activities of SOD and POD of the three FT groups all showed a negative correlation with temperature. The MDA showed a significant correlation with other indicators, and the correlations among the other above physiological indexes were not significant (p< 0.05). The combined stress of FT + DA can cause increased plant damage, such as lipid peroxidation and osmotic potential imbalance. As an environmentally friendly DA, concentrated CMA was less harmful to plants than sodium chloride DA, but still brought damage to plants inevitably. Our results and corresponding discussion highlight the new findings on the effects of two kinds of deicing agents on plants under FT stress.