Cloning of cDNA and analysis of the gene for mouse angiotensin II type 2 receptor.
Cloning of cDNA and analysis of the gene for mouse angiotensin II type 2 receptor.
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DOI：
10.1006/bbrc.1993.2492
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发表时间：
1993-12
影响因子：
3.1
通讯作者：
M. Nakajima;M. Mukoyama;R. Pratt;M. Horiuchi;V. Dzau
中科院分区：
文献类型：
--
作者：
M. Nakajima;M. Mukoyama;R. Pratt;M. Horiuchi;V. Dzau
从小鼠胎儿cDNA文库中分离出编码小鼠血管紧张素受体亚型2（AT 2受体）的cDNA克隆。小鼠AT 2受体全长cDNA包含一个1089个碱基的开放阅读框，编码363个氨基酸残基的蛋白质。亲水性分析揭示了七个推定的跨膜结构域。[125 I] CGP 42112 A特异性结合在转染cDNA的COS-7细胞膜中。Scatchard分析和配体置换曲线是AT 2受体的典型特征。北方杂交和逆转录-聚合酶链反应分析表明，AT 2受体mRNA在胎儿脑中大量表达，而在成人脑中表达较少。对小鼠AT 2受体基因的分析揭示，小鼠AT 2受体基因座在基因组中以单拷贝存在，并且该基因在编码区中不含内含子。
A cDNA clone encoding the mouse angiotensin receptor subtype 2 (AT2 receptor) was isolated from a mouse fetal cDNA library. Mouse AT2 receptor full-length cDNA contains a 1089-base-pair open reading frame which encodes a protein of 363 amino acid residues. Hydropathy analysis reveals seven putative transmembrane domains. Specific binding of [125I]CGP42112A was demonstrated in membranes of COS-7 cells transfected with the cDNA. Scatchard analysis and ligand displacement profile were typical of the AT2 receptor. Northern blot and reverse transcription-polymerase chain reaction analyses demonstrated that AT2 receptor mRNA was expressed abundantly in fetus and in a lesser amount in adult brain. Analysis of the mouse AT2 receptor gene reveals that the mouse AT2 receptor locus exists as a single copy in the genome, and that the gene does not contain introns in the coding region.