ANTIBACTERIAL AND HEMOLYTIC PEPTIDES CONTAINING D-ALLOISOLEUCINE FROM THE SKIN OF BOMBINA-VARIEGATA
ANTIBACTERIAL AND HEMOLYTIC PEPTIDES CONTAINING D-ALLOISOLEUCINE FROM THE SKIN OF BOMBINA-VARIEGATA
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DOI：
10.1002/j.1460-2075.1993.tb06172.x
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发表时间：
1993-12-01
期刊：
影响因子：
11.4
通讯作者：
BARRA, D
中科院分区：
文献类型：
--
作者：
MIGNOGNA, G;SIMMACO, M;BARRA, D
Bombina varegata的皮肤中存在一个与Bombinin相关的多肽家族。这些多肽含有27个氨基酸残基，N端为甘氨酸，具有抗菌活性。从编码相应多肽前体的cDNA的序列分析中，预测到一个新的以Ile为N末端的20个残基的多肽的存在。我们现在已经纯化了一系列疏水性多肽，命名为H1-H5，其序列与预测的多肽相对应，在第1、2和8位有一定的变异性。特别是，H3-H5在第二位含有D-异亮氨酸残基。所有这些多肽都具有抗菌和溶血活性。
A family of bombinin-related peptides is present in the skin of Bombina variegata. These peptides contain 27 residues with Gly as N-terminus and display antimicrobial activity. From sequence analysis of the cDNAs encoding for the corresponding peptide precursors, the presence of a novel 20-residue peptide with Ile as N-terminus was predicted. We have now purified a family of hydrophobic peptides named H1-H5, whose sequences correspond to the predicted peptide with some variability in positions 1, 2 and 8. In particular, H3-H5 contain a D-alloisoleucine residue in the second position. All these peptides display antibacterial and haemolytic activity.