A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice
A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice
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DOI：
10.1534/genetics.104.034801
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发表时间：
2004-10-01
期刊：
影响因子：
3.3
通讯作者：
Anderson, JA
中科院分区：
文献类型：
--
作者：
Conley, EJ;Nduati, V;Anderson, JA
复杂的小麦六倍体基因组为基因组学研究提出了许多挑战。表达的序列标签有助于基因编码区域的分析，并为模式生物的定位和比较提供了丰富的分子标记来源。本研究的目的是构建小麦同源第2组染色体的高密度EST染色体库图，以确定EST的分布，构建第2组EST的一致性图，研究第2组EST的同质性，检查重复模式，并评估第2组EST与水稻的共线性。通过对1110个est序列的Southern杂交，将2600个基因座定位到第2组染色体上。将552条ESTs与一个以上的2组染色体进行比对，构建了一致图谱。发现远端箱区基因密度高，近端箱区基因密度低。在短臂和长臂上发现了两个基因丰富的间质岛，其两侧是基因相对贫乏的区域，与水稻具有良好的同源性。两个ESTs的图谱位置提示2B染色体可能存在小的中心反转。小麦第2组染色体与水稻第4和第7组染色体具有同源块。
The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chromosome bin map of wheat homoeologous group 2 chromosomes to determine the distribution of ESTs, construct a consensus map of group 2 ESTs, investigate synteny, examine patterns of duplication, and assess the colinearity with rice of ESTs assigned to the group 2 consensus bin map. A total of 2600 loci generated from 1110 ESTs were mapped to group 2 chromosomes by Southern hybridization onto wheat aneuploid chromosome and deletion stocks. A consensus map was constructed of 552 ESTs mapping to more than one group 2 chromosome. Regions of high gene density in distal bins and low gene density in proximal bins were found. Two interstitial gene-rich islands flanked by relatively gene-poor regions on both the short and long arms and having good synteny with rice were discovered. The map locations of two ESTs indicated the possible presence of a small pericentric inversion on chromosome 2B. Wheat chromosome group 2 was shown to share synteneous blocks with rice chromosomes 4 and 7.