Effects of antibacterial peptide-producing Bacillus subtilis and Lactobacillus buchneri on fermentation, aerobic stability, and microbial community of alfalfa silage
Effects of antibacterial peptide-producing Bacillus subtilis and Lactobacillus buchneri on fermentation, aerobic stability, and microbial community of alfalfa silage
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产抗菌肽枯草芽孢杆菌和布氏乳杆菌对苜蓿青贮饲料发酵、有氧稳定性和微生物群落的影响
DOI：
10.1016/j.biortech.2020.123881
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发表时间：
2020-11-01
影响因子：
11.4
通讯作者：
Guo, Xusheng
中科院分区：
文献类型：
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作者：
Bai, Jie;Xu, Dongmei;Guo, Xusheng
本研究探讨了枯草芽孢杆菌（BS）、布氏乳杆菌（LB）及其组合对苜蓿青贮发酵、蛋白水解、有氧稳定性和微生物群落的影响。结果表明，与对照组相比，BS处理的青贮饲料乳酸含量较高，蛋白质水解较少，有氧稳定性较高。LB和BS处理增加了乳酸菌和壳二胞的丰度，并降低了肠球菌和孢子菌的丰度后60天的发酵。LB和BS在好氧暴露3 d后也抑制了肠球菌的生长，但与对照青贮相似，BS青贮在好氧暴露9 d后真菌群落以假丝酵母和毕赤酵母为主。因此，接种BS可以改善青贮期间和有氧暴露3d后的青贮发酵品质、有氧稳定性和细菌群落。
This study assessed the effects of antibacterial peptide-producing Bacillus subtilis (BS), Lactobacillus buchneri (LB), or their combination on fermentation, proteolysis, aerobic stability, and microbial communities during ensiling and aerobic exposure phases of alfalfa silage. The results showed that the BS-treated silage displayed a higher lactic acid concentration, less proteolysis, and higher aerobic stability than those in the control silage. Both LB and BS treatments increased Lactobacillus and Ascochyta abundance, and decreased Enterococcus and Sporormiacea abundance after 60 d of fermentation. LB and BS also inhibited the growth of Enterococcus after 3 d of aerobic exposure but similar to the control silage, the fungal community of BS silage was dominated by Candida and Pichia after 9 d of aerobic exposure. Therefore, inoculation of BS improved silage fermentation quality, aerobic stability and bacterial community during ensiling and after 3 d of aerobic exposure.