INTERCALATING DRUGS AND LOW-TEMPERATURES INHIBIT SYNTHESIS AND PROCESSING OF RIBOSOMAL-RNA IN ISOLATED HUMAN MITOCHONDRIA
INTERCALATING DRUGS AND LOW-TEMPERATURES INHIBIT SYNTHESIS AND PROCESSING OF RIBOSOMAL-RNA IN ISOLATED HUMAN MITOCHONDRIA
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DOI：
10.1016/s0022-2836(84)80017-0
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发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
ATTARDI, G
中科院分区：
文献类型：
--
作者：
GAINES, G;ATTARDI, G
分离的HeLa细胞线粒体中的线粒体DNA转录忠实地再现了体内过程。放线菌素D、原黄素和溴化乙锭优先抑制rRNA的形成，而不是mRNA的形成，强烈支持参与其合成的2个重叠转录单位的独立控制。去除rRNA前体5“末端的tRNAPhe序列的加工步骤对低温、原黄素和溴化乙锭独特敏感。
Mitochondrial DNA transcription in isolated HeLa cell mitochondria faithfully reproduces the in vivo process. Actinomycin D, proflavine and ethidium bromide preferentially inhibit the formation of rRNA over that of mRNA, strongly supporting independent controls of the 2 overlapping transcription units involved in their synthesis. The processing step removing the tRNAPhe sequence at the 5'' end of the rRNA precursor is uniquely sensitive to low temperature, proflavine and ethidium bromide.