Relationship between cartilage and subchondral bone lesions in repetitive impact trauma-induced equine osteoarthritis
Relationship between cartilage and subchondral bone lesions in repetitive impact trauma-induced equine osteoarthritis
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DOI：
10.1016/j.joca.2012.02.004
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发表时间：
2012-06-01
影响因子：
7
通讯作者：
Laverty, S.
中科院分区：
文献类型：
--
作者：
Lacourt, M.;Gao, C.;Laverty, S.
目的：探讨标准种马第三腕骨(C3)软骨和软骨下骨的退行性变化与自然发生的反复创伤性骨关节炎的关系。设计：对15匹标准种马身体标本进行软骨损伤的肉眼观察，分为对照组(CO)、早期骨关节炎(EOA)和晚期骨关节炎(AOA)组。从每根骨的相应背侧部位取两个骨软骨核，用微型计算机断层扫描仪(CT)扫描。2D图像被组装成3D重建，用于量化选定感兴趣区域的建筑参数，包括骨密度和骨体积分数。2D图像显示了每个核心最严重的病变，盲人观察者对建筑外观进行了评分。用改良的Mankin评分系统对石蜡包埋的脱钙核石蜡切片进行评分。结果：软骨下骨陷窝在AOA组比EOA组更多见，但在CO组中未见。关节软骨损伤与骨矿物质减少和骨组织丢失有关。组织学分析显示，与CO骨相比，EOA组和AOA组的钙化软骨中有大量的微裂缝，且评分呈进行性增加。结论：这些数据显示，在重复、高冲击性创伤中，赛马第三腕骨的关节软骨和软骨下骨发生了相应的进行性退行性改变，包括明显的局灶性吸收损害。(C)2012年国际骨性关节炎研究会。爱思唯尔有限公司出版。保留所有权利。
Objective: To correlate degenerative changes in cartilage and subchondral bone in the third carpal bone (C3) of Standardbred racehorses with naturally occurring repetitive trauma-induced osteoarthritis.Design: Fifteen C3, collected from Standardbred horses postmortem, were assessed for cartilage lesions by visual inspection and divided into Control (CO), Early Osteoarthritis (EOA) and Advanced Osteoarthritis (AOA) groups. Two osteochondral cores were harvested from corresponding dorsal sites on each bone and scanned with a micro-computed tomography (CT) instrument. 2D images were assembled into 3D reconstructions that were used to quantify architectural parameters from selected regions of interest, including bone mineral density and bone volume fraction. 2D images, illustrating the most severe lesion per core, were scored for architectural appearance by blinded observers. Thin sections of paraffin embedded decalcified cores stained with Safranin O-Fast Green, matched to the micro-CT images, were scored using a modified Mankin scoring system.Results: Subchondral bone pits with deep focal areas of porosity were seen more frequently in AOA than EOA but never in CO. Articular cartilage damage was seen in association with a reduction in bone mineral and loss of bone tissue. Histological analyses revealed significant numbers of microcracks in the calcified cartilage of EOA and AOA groups and a progressive increase in the score compared with CO bones.Conclusion: The data reveal corresponding, progressive degenerative changes in articular cartilage and subchondral bone, including striking focal resorptive lesions, in the third carpal bone of racehorses subjected to repetitive, high impact trauma. (C) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.