Increased reticulocyte count from cord blood samples using hypotonic lysis
Increased reticulocyte count from cord blood samples using hypotonic lysis
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DOI：
10.1016/j.exppara.2012.07.006
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发表时间：
2012-10-01
影响因子：
2.1
通讯作者：
Zimmerman, Peter A.
中科院分区：
文献类型：
--
作者：
Grimberg, Brian T.;Scheetz, Emily A.;Zimmerman, Peter A.
人类网织红细胞是研究人类疟原虫间日疟原虫体外侵袭过程所需的基本成分之一。此外，在世界上无法获得先进设备或干细胞系的地区，网织红细胞及其结合蛋白的检查很困难。这些问题与疟疾候选疫苗研究特别相关，这些疫苗针对寄生虫用来进入红细胞的表面蛋白。这里描述的是一种增加脐带血样本网织红细胞计数的简单且廉价的方法。将脐带血暴露于低渗盐水 (0.2%) 5 分钟，选择性裂解非网织红细胞，导致网织红细胞计数平均增加 3.6 倍。我们的研究表明，这种富集过程不会损害剩余红细胞的血红蛋白，这些红细胞仍然能够支持恶性疟原虫的入侵和生长。这种经济且快速的富集方法可以促进对优先侵入网织红细胞（例如间日疟原虫）的其他疟疾寄生虫物种的体外实验室培养的研究。 (c) 2012 年，爱思唯尔公司出版。
Human reticulocytes are one of the fundamental components needed to study the in vitro invasion processes of the human malaria parasite Plasmodium vivax. Additionally examinations of reticulocytes and their binding proteins are difficult in areas of the world that do not have access to advanced equipment or stem cell lines. These issues are particularly relevant to malaria vaccine candidate studies that are directed against surface proteins that the parasites use to gain entry into erythrocytes. Described here is a simple and inexpensive method to increase the reticulocyte count of cord blood samples. Exposure of cord blood to hypotonic saline (0.2%) for 5 min selectively lyses the non-reticulocytes resulting in an average 3.6-fold increase in reticulocyte count. Our studies show that this enrichment process does not damage the hemoglobin of the remaining erythrocytes which are still capable of supporting Plasmodium falciparum invasion and growth. This economical and rapid method of enrichment could facilitate studies of in vitro laboratory culturing of other malaria parasite species which preferentially invade reticulocytes such as P. vivax. (c) 2012 Published by Elsevier Inc.