The chemoattractant activity of rheumatoid synovial fluid for human lymphocytes is due to multiple cytokines
The chemoattractant activity of rheumatoid synovial fluid for human lymphocytes is due to multiple cytokines
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DOI：
10.1046/j.1365-2249.1996.d01-836.x
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发表时间：
1996-11-01
影响因子：
4.6
通讯作者：
Wilkinson, PC
中科院分区：
文献类型：
--
作者：
AlMughales, J;Blyth, TH;Wilkinson, PC
29例类风湿关节炎患者的滑膜液对正常血液淋巴细胞具有很强的吸引力。虽然类风湿性滑膜液含有IL-15、IL-8、单核细胞趋化蛋白-1 （MCP-1）和巨噬细胞炎性蛋白-1 α (MIP-1 α)，其水平足以吸引淋巴细胞，但使用特异性抗体抑制任何单一细胞因子的活性并不能消除液体的活性。然而，联合使用抗细胞因子抗体（抗il -15+抗mcp -1；抗il -8+抗mcp -1或+抗mip -1 α）抑制了大部分活性，这表明淋巴细胞被液体吸引是由于引诱剂的组合。血液淋巴细胞需要通过夜间培养激活才能达到最佳反应，而类风湿滑膜组织淋巴细胞对滑膜液有反应而不需要一段时间的培养。来自类风湿性滑膜液的淋巴细胞对运动兴奋剂反应较差。血单核细胞反应细胞多为CD45RO同型T淋巴细胞。存在环孢素A或皮质类固醇的孵育抑制淋巴细胞对液体的反应，但存在非甾体抗炎药（NSAIDs）和其他用于治疗患者的药物对液体没有抑制作用。
The majority of synovial fluids from 29 rheumatoid arthritis patients were strongly attractive for normal blood lymphocytes judged by assays of polarization and collagen gel invasion. While rheumatoid synovial fluids contained IL-15, IL-8, monocyte chemotactic protein-1 (MCP-1) and macrophage inflammatory protein-1 alpha (MIP-1 alpha) at levels sufficient to attract lymphocytes, inhibition of the activity of any single cytokine using specific antibody did not abolish the activity of the fluid. However combinations of anti-cytokine antibodies used together (anti-IL-15+anti-MCP-1; anti-IL-8+anti-MCP-1 or +anti-MIP-1 alpha) inhibited most of the activity, suggesting that attraction of lymphocytes by the fluids is due to a combination of attractants. Blood lymphocytes required activation by overnight culture to respond optimally, while rheumatoid synovial tissue lymphocytes responded to synovial fluids without a requirement for a period of culture. Lymphocytes derived from rheumatoid synovial fluids were poorly responsive to locomotor stimulants. Most of the responding cells from blood mononuclear cell fractions were T lymphocytes of the CD45RO isotype. Incubation in the presence of cyclosporin A or corticosteroids inhibited the response of lymphocytes to the fluids, but the presence of non-steroidal anti-inflammatory drugs (NSAIDs) and other agents used in therapy of the patients from whom the fluids were taken had no inhibitory effect.