Transcription of [FeFe]-Hydrogenase Genes during H(2) Production in Clostridium and Desulfovibrio spp. Isolated from a Paddy Field Soil.
Transcription of [FeFe]-Hydrogenase Genes during H(2) Production in Clostridium and Desulfovibrio spp. Isolated from a Paddy Field Soil.
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DOI：
10.1264/jsme2.me16171
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发表时间：
2017-06-24
影响因子：
2.2
通讯作者：
Watanabe T
中科院分区：
文献类型：
--
作者：
Baba R;Morita M;Asakawa S;Watanabe T
分析了水田土壤中分离的Clostridium sp. H2和Desulfovibrio sp. A1菌株在产氢过程中[FeFe]-氢化酶hydA基因相似物的相对丰度变化。菌株H2和A1分别具有至少5个和2个不同的hydA基因。不同种类的水合酶转录物相对丰度不同，产氢活性随生长阶段和条件的不同而变化。菌株H2中5个hydA基因中2个转录本相对丰度的升高或降低与产氢速率的变化相关，其余转录本相对丰度不变或降低。在菌株A1中，两个hydA基因转录本的相对丰度在单一培养、硫酸盐还原和共生产甲烷条件下有所不同。其中一个编码胞质[FeFe]氢化酶的hydA基因的相对丰度在合成气和产甲烷条件下比硫酸盐还原条件下更高，而另一个hydA基因的相对丰度在这两种条件下都随着时间的推移而降低。本研究表明，水田土壤中分离的产氢菌株在产氢过程中，hydA基因的转录受到不同的调控。
Changes in the relative abundances of the transcripts of hydA gene paralogs for [FeFe]-hydrogenase in Clostridium sp. strain H2 and Desulfovibrio sp. strain A1 isolated from paddy field soil were analyzed during H2 production. Strains H2 and A1 had at least five and two phylogenetically different hydA genes, respectively. The relative abundances of their hydA transcripts differed among the paralogs and H2 production activity changed in a manner that depended on the growth phase and conditions. Increases or decreases in the relative abundances of the transcripts of two out of five hydA genes in strain H2 correlated with changes in H2 production rates, whereas those of the others remained unchanged or decreased. In strain A1, the relative abundances of the transcripts of two hydA genes differed between monoculture, sulfate-reducing, and syntrophic, methanogenic conditions. The relative abundance of the transcripts of one hydA gene, predicted to encode a cytosolic [FeFe]-hydrogenase, was higher under syntrophic, methanogenic conditions than sulfate-reducing conditions, while that of the transcripts of the other hydA gene decreased with time under both conditions. This study showed that the transcription of the hydA gene during growth with active H2 production was differently regulated among the paralogs in H2 producers isolated from paddy field soil.