Light attenuates lipid accumulation while enhancing cell proliferation and starch synthesis in the glucose-fed oleaginous microalga Chlorella zofingiensis.
Light attenuates lipid accumulation while enhancing cell proliferation and starch synthesis in the glucose-fed oleaginous microalga Chlorella zofingiensis.
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光减弱了葡萄糖喂养的产油微藻 Chlorella zofingiensis 中的脂质积累，同时增强了细胞增殖和淀粉合成
DOI：
10.1038/srep14936
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发表时间：
2015-10-07
影响因子：
4.6
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Chen T;Liu J;Guo B;Ma X;Sun P;Liu B;Chen F
本研究的目的是研究光照对产油绿藻小球藻（Chlorella zoofingiensis）体内脂质和淀粉积累的影响。zofingiensis在添加30 g/L葡萄糖时，合成的脂质最高可达0.531 g/g干重；光照条件下，脂质含量降至0.352 g/g干重。葡萄糖的脂质产量为0.184 g/g葡萄糖，比光培养高14%。光介导的脂质减少在转录水平上伴随着脂肪酸生物合成基因的下调。此外，光促进细胞增殖，淀粉积累和淀粉产量的葡萄糖。综上所述，光可能通过抑制脂质生物合成途径减弱脂质积累，导致更多的碳从葡萄糖到淀粉的通量。本研究揭示了光对糖食性zofingiensis的双重影响，并为通过操纵培养条件优化藻类生物量和脂质生产提供了有价值的见解。
The objective of this study was to investigate the effect of light on lipid and starch accumulation in the oleaginous green algae Chlorella zofingiensis supplemented with glucose. C. zofingiensis, when fed with 30 g/L glucose, synthesized lipids up to 0.531 g/g dry weight; while in the presence of light, the lipid content dropped down to 0.352 g/g dry weight. Lipid yield on glucose was 0.184 g/g glucose, 14% higher than that cultured with light. The light-mediated lipid reduction was accompanied by the down-regulation of fatty acid biosynthetic genes at the transcriptional level. Furthermore, light promoted cell proliferation, starch accumulation and the starch yield based on glucose. Taken together, light may attenuate lipid accumulation, possibly through the inhibition of lipid biosynthetic pathway, leading to more carbon flux from glucose to starch. This study reveals the dual effects of light on the sugar-fed C. zofingiensis and provides valuable insights into the possible optimization of algal biomass and lipid production by manipulation of culture conditions.