DNA molecules in microfluidic oscillatory flow
DNA molecules in microfluidic oscillatory flow
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DOI：
10.1021/ma050238d
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发表时间：
2005-07-26
期刊：
影响因子：
5.5
通讯作者：
Schwartz, DC
中科院分区：
文献类型：
--
作者：
Chen, YL;Graham, MD;Schwartz, DC
使用布朗动力学模拟研究了在微流体通道中进行振荡压力驱动流动的单个DNA分子的构象和动力学，考虑了块体中片段之间以及链与壁之间的流体动力学相互作用。振荡流提供了一种情况，在这种情况下，聚合物可以在通道中保持不确定的时间，因为它们被拉伸并远离通道壁迁移。我们表明，通过控制链的长度，流速，和振荡流频率，我们能够操纵链的延伸和链迁移从通道壁。链拉伸和链耗尽层厚度附近的壁被发现增加作为Weissenberg数的增加和振荡频率的降低。
The conformation and dynamics of a single DNA molecule undergoing oscillatory pressure-driven flow in microfluidic channels are studied using Brownian dynamics simulations, accounting for hydrodynamic interactions between segments in the bulk and between the chain and the walls. Oscillatory flow provides a scenario under which the polymers may remain in the channel for an indefinite amount Of time as they are stretched and migrate away from the channel walls. We show that by controlling the chain length, flow rate, and oscillatory flow frequency we are able to manipulate the chain extension and the chain migration from the channel walls. The chain stretch and the chain depletion layer thickness near the wall are found to increase as the Weissenberg number increases and as the oscillatory frequency decreases.