High glucose induces antioxidant enzymes in human endothelial cells in culture - Evidence linking hyperglycemia and oxidative stress
High glucose induces antioxidant enzymes in human endothelial cells in culture - Evidence linking hyperglycemia and oxidative stress
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DOI：
10.2337/diabetes.45.4.471
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发表时间：
1996-04-01
期刊：
影响因子：
7.7
通讯作者：
Cerutti, P
中科院分区：
文献类型：
--
作者：
Ceriello, A;delloRusso, P;Cerutti, P
氧化应激可能在糖尿病并发症的发病机制中起重要作用，高血压可能导致自由基产生增加，并且证据支持这些反应性分子作为糖尿病内皮细胞功能障碍的介质的突出作用。已经证明，活性氧物质诱导某些组织中的抗氧化酶表达，并且这种现象被认为是存在氧依赖性毒性的证据。在这项研究中，人脐静脉内皮细胞，永生化的人内皮细胞，永生化的人内皮细胞转染表达高谷胱甘肽过氧化物酶水平生长在正常和高糖条件下。高糖延迟复制后7和14天的人内皮细胞培养，无论是从脐静脉和永生化，而转染的细胞不受影响。抗氧化酶CuZn-超氧化物歧化酶、Mn-超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性和mRNA表达在培养2、7和14天后进行评价。高糖在第7天和第14天诱导的过度表达CuZn-超氧化物歧化酶，过氧化氢酶，谷胱甘肽过氧化物酶在人脐静脉内皮细胞和永生化的人内皮细胞，而在转染细胞中，它没有。这项研究表明，高葡萄糖诱导人内皮细胞中抗氧化酶水平的增加，表明葡萄糖水平升高可能会在细胞中产生氧化应激。
It has been suggested that oxidative stress may play an important role in the pathogenesis of diabetic complications, Hyperglycemia may cause increased production of free radicals, and evidence supports a prominent role for these reactive molecules as mediators of endothelial cell dysfunction in diabetes. It has been demonstrated that active oxygen species induce antioxidant enzyme expression in some tissues, and this phenomenon is considered proof of an existing oxygen-dependent toxicity. In this study, human endothelial cells from umbilical vein, immortalized human endothelial cells, and immortalized human endothelial cells transfected to express high glutathione peroxidase levels were grown in normal and high-glucose conditions. High glucose delayed replication after 7 and 14 days of culture of human endothelial cells, both from umbilical vein and immortalized, while transfected cells were not affected. The activity and the mRNA expression of tile antioxidant enzymes CuZn-superoxide-dismutase, Mn-superoxide-dismutase, catalase, and glutathione peroxidase were evaluated after 2, 7, and 14 days of culture. High glucose at days 7 and 14 induced an overexpression of CuZn-superoxide-dismutase, catalase, and glutathione peroxidase in both human endothelial cells from umbilical vein and immortalized human endothelial cells, while in transfected cells it did not. This study demonstrates that high glucose induces an increase in antioxidant enzyme levels in human endothelial cells, suggesting that elevated glucose levels may produce an oxidative stress in the cells.