Lipotoxicity: impact on oocyte quality and reproductive efficiency in mammals
Lipotoxicity: impact on oocyte quality and reproductive efficiency in mammals
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脂毒性：对哺乳动物卵母细胞质量和生殖效率的影响
DOI：
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
D. Rondina
中科院分区：
文献类型：
--
作者：
J. P. Alves;M. Bertolini;L. Bertolini;C.M.G. Silva;D. Rondina
脂毒性的特征在于血液中过量的饱和脂肪酸，增加非脂肪细胞中的储存，这导致与内质网应激相关的基因的表达模式的变化（例如，ATF 4、ATF 6、CHOP和GRP 78）、促和抗凋亡途径（例如，Bax和Bcl-2，以及蛋白质稳定性，包括热休克蛋白，例如，HSP 70）。负亚细胞效应通常是最终结果，其也发生在卵巢卵泡群体中，影响颗粒细胞和卵丘-卵母细胞复合体（COC），这导致卵母细胞质量和线粒体活性降低，以及细胞凋亡增加。向卵母细胞体外成熟培养基中添加高剂量的非酯化脂肪酸已显示减缓减数分裂的进展，阻碍卵母细胞成熟和随后的体外胚胎发育。由于其在控制细胞脂滴中的重要性以及与胞质脂质积累的表达相关性，Plin 2（Perilipin 2）蛋白的表达也被强调。本文综述了反刍动物雌性日粮中添加脂类对生殖的影响、脂类毒性对卵母细胞质量和生殖的潜在影响以及内质网应激和细胞脂质积累相关基因表达的主要机制。
Lipotoxicity is characterized by excessive saturated fatty acids in the blood, increasing storage in non-adipose cells, which leads to changes in the expression pattern of genes related to endoplasmic reticulum stress (e.g., ATF4, ATF6, CHOP, and GRP78), pro- and anti-apoptotic pathways (e.g., Bax and Bcl-2, and protein stability, including heat shock proteins, e.g., HSP70). A negative sub-cellular effect is usually an end result, which also occurs in the ovarian follicular population, affecting granulosa cells and cumulus-oocyte complexes (COCs), which leads to a decrease in oocyte quality and mitochondrial activity, and increased apoptosis. The addition of high doses of non-esterified fatty acids to oocyte in vitro maturation medium has been shown to slow the progression of meiosis, hampering oocyte maturation and subsequent in vitro embryo development. Due to its importance in the control of cellular lipid droplets and expression correlation with cytosolic lipid accumulation, the expression of the Plin 2 (Perilipin 2) protein is also highlighted. The aim of this review is to discuss some reproductive implications of dietary lipid supplementation in ruminant females, and the potential effects of lipotoxicity on oocyte quality and reproduction, and the main mechanisms involved in the expression of genes related to endoplasmic reticulum stress and cellular lipid accumulation.
影响因子：
10.5
作者：
Zinszner, H;Kuroda, M;Ron, D
通讯作者：
Ron, D
影响因子：
16
作者：
Ye, J;Rawson, RB;Goldstein, JL
通讯作者：
Goldstein, JL
影响因子：
4.8
作者：
Jungheim, Emily S.;Schoeller, Erica L.;Moley, Kelle H.
通讯作者：
Moley, Kelle H.