Superparamagnetic iron oxide-based method for quantifying recruitment of monocytes to mouse atherosclerotic lesions in vivo -: Enhancement by tissue necrosis factor-α, interleukin-1β, and interferon-γ
Superparamagnetic iron oxide-based method for quantifying recruitment of monocytes to mouse atherosclerotic lesions in vivo -: Enhancement by tissue necrosis factor-α, interleukin-1β, and interferon-γ
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DOI：
10.1161/01.cir.0000055323.57885.88
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发表时间：
2003-03-25
期刊：
影响因子：
37.8
通讯作者：
Naghavi, M
中科院分区：
文献类型：
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作者：
Litovsky, S;Madjid, M;Naghavi, M
背景-最近发现MRI造影剂超顺磁性氧化铁(SPIO)定位于主动脉粥样硬化斑块。因此，我们询问 SPIO 是否可用于监测主动脉粥样硬化斑块中单核细胞的募集。方法和结果 - 将 11 只雌性 apo E 敲除 (K/O) 小鼠，每只 11 个月大，分为 2 组。六只小鼠接受组织坏死因子-α（0.2杯IP一次）、白细胞介素-1β（0.2杯IP一次）和干扰素-γ（每天100U/gIP，持续5天）； 5 名受试者接受 0.5 mL 含有 1% BSA 的生理盐水，并作为假治疗的动脉粥样硬化对照。两只野生型 C57BL/6 小鼠作为假处理的非动脉粥样硬化对照。初始细胞因子或假治疗后三小时，所有小鼠通过静脉注射接受 SPIO（1 mmol/kg 铁）。六天后，对所有小鼠实施安乐死，在生理压力下对心脏和主动脉进行灌注，并对整个主动脉进行组织学研究。与假治疗的 apo E K/O 对照小鼠相比，接受细胞因子治疗的小鼠的动脉粥样硬化斑块每个横截面含有更多的铁阳性巨噬细胞（42+/-11.8 对比 11.6+/-5.9）（P
Background-It has been found recently that the MRI contrast agent superparamagnetic iron oxide ( SPIO) localizes to aortic atherosclerotic plaques. We therefore asked whether SPIO might be used to monitor monocyte recruitment into aortic atherosclerotic plaques.Methods and Results-Eleven female apo E knockout (K/O) mice, each 11 months old, were divided into 2 groups. Six mice received tissue necrosis factor-alpha (0.2 mug IP once), interleukin-1beta (0.2 mug IP once), and interferon-gamma (100 U/g per day IP for 5 days); 5 received 0.5 mL saline containing1% BSA and served as sham-treated atherosclerotic controls. Two wild-type C57BL/6 mice served as sham-treated nonatherosclerotic controls. Three hours after initial cytokine or sham treatment, all mice received SPIO by intravenous injection (1 mmol/kg iron). Six days later, all mice were euthanized, the hearts and aortas were perfused under physiological pressure, and the entire aortas were studied histologically. Atherosclerotic plaques in cytokine-treated mice contained more iron-positive macrophages per cross section than did those in sham-treated apo E K/O control mice (42+/-11.8 versus 11.6+/-5.9) (P