Fluorescence-based method, exploiting lipofuscin, for real-time detection of central nervous system tissues on bovine carcasses.
Fluorescence-based method, exploiting lipofuscin, for real-time detection of central nervous system tissues on bovine carcasses.
复制标题
基于荧光的方法，利用脂褐质，实时检测牛尸体上的中枢神经系统组织。
DOI：
10.1021/jf0734368
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发表时间：
2008
影响因子：
6.1
通讯作者：
J. Petrich
中科院分区：
文献类型：
--
作者：
H. Schönenbrücher;Ramkrishna Adhikary;P. Mukherjee;T. Casey;M. Rasmussen;Frank D Maistrovich;A. Hamir;M. Kehrli;J. Richt;J. Petrich
去除作为牛海绵状脑病（BSE）风险材料一部分的中枢神经系统（CNS）组织是避免疯牛病污染人类食物链的最优先任务之一。目前没有可用的方法能够实时检测屠宰期间胴体上可能的中枢神经系统组织污染。荧光色素脂褐素是一种异质性、高分子量的物质，已被证明在神经元组织中高浓度富集。在本研究中，脂褐素荧光作为实时检测中枢神经系统污染的标记物进行了研究。11头牛的大脑和脊髓样品的正面荧光光谱显示出相同的、可重复的高强度荧光信号模式。通过研究13种不同的非中枢神经系统组织，分别通过信号强度和光谱结构来区分脑和脊髓，评估这些光谱的特异性。少量牛脊髓在生牛骨骼肌、脂肪和椎骨的存在下被可靠地检测到。提出的数据是一个基本的基础，为原型设备的发展，允许实时监测中枢神经系统组织污染的牛尸体和肉切割。
The removal of central nervous system (CNS) tissues as part of bovine spongiform encephalopathy (BSE) risk material is one of the highest priority tasks to avoid contamination of the human food chain with BSE. No currently available method enables the real-time detection of possible CNS tissue contamination on carcasses during slaughter. The fluorescent pigment lipofuscin is a heterogeneous, high-molecular weight material that has been shown to be enriched in high concentrations in neuronal tissues. In this study, lipofuscin fluorescence was investigated as a marker for real-time detection of CNS contamination. Front-faced fluorescence spectra of brain and spinal cord samples from 11 cattle gave identical, reproducible fluorescence signal patterns with high intensities. The specificity of these spectra was assessed by investigating 13 different non-CNS tissues enabling the differentiation of brain and spinal cord by signal intensity and structure of the spectra, respectively. Small quantities of bovine spinal cord were reliably detected in the presence of raw bovine skeletal muscle, fat, and vertebrae. The presented data are a fundamental basis for the development of a prototype device allowing real-time monitoring of CNS tissue contamination on bovine carcasses and meat cuts.