A Novel β-Glucosidase From Chryseobacterium scophthalmum 1433 for Efficient Rubusoside Production From Stevioside.
A Novel β-Glucosidase From Chryseobacterium scophthalmum 1433 for Efficient Rubusoside Production From Stevioside.
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来自Scophthalmum 1433的新型β-葡萄糖苷酶，用于从甜叶菊产生有效的Rubusoside。
DOI：
10.3389/fmicb.2021.744914
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发表时间：
2021
影响因子：
5.2
通讯作者：
Xiao M
中科院分区：
文献类型：
--
作者：
Yan Z;Cao X;Yang X;Yang S;Xu L;Jiang X;Xiao M
作为一种天然的增甜剂和增溶剂，冬凌草苷在健康饮料和药品中具有很大的应用潜力。然而，从原料中直接提取和纯化冬凌草苷是低效的。本研究通过对环境微生物的筛选，从scophthalmum Chryseobacterium 1433中获得了一种新的β-葡萄糖苷酶（CsBGL）。CsBGL对苦参糖（Glcβ1- 2glc）和薄层糖（Glcβ1- 3glc）有明显的水解作用，但对于甜菊醇苷，它只能水解C-13/ c -19连接的苦参糖，而不能水解C-13/ c -19连接的Glcβ1-2[Glcβ1-3]Glc三糖和Glcβ1-单糖。在47.5℃、70 min条件下高效水解甜菊糖苷（240 g/L），产率99%。即使使用含有~ 226 g/L甜菊糖苷的甜菊糖苷粗提取物（500 g/L）作为底物，CsBGL也能在47.5℃条件下将甜菊糖苷转化为苦菊糖苷（99%收率）2 h，苦菊糖苷浓度从最初的42 g/L增加到最终的222 g/L。这些结果表明，CsBGL是一种很有前景的生物催化剂，可用于从甜菊糖苷或甜菊糖苷粗提取物中工业化生产甜菊糖苷。作为一种天然的增甜剂和增溶剂，冬凌草苷在健康饮料和药品中具有很大的应用潜力。本文通过筛选环境微生物，从scophthalmum Chryseobacterium 1433中获得了一种新的β-葡萄糖苷酶CsBGL，该酶能特异性水解甜菊糖苷的C-13-或/和c -19连接的苦参糖，但不能水解甜菊糖苷的C-13-或/和c -19连接的Glcβ1-2[Glcβ1-3]Glc三糖和Glcβ1-单糖，因此可能是一种很有前景的从甜菊糖苷高效生产甜菊糖苷的生物催化剂。
As a natural sweetening and solubilizing agent, rubusoside has great potential in the application of healthy beverages and pharmaceuticals. However, the direct extraction and purification of rubusoside from raw materials is inefficient. In this work, a novel β-glucosidase (CsBGL) was obtained from Chryseobacterium scophthalmum 1433 through screening of the environmental microorganisms. CsBGL markedly hydrolyzed sophorese (Glcβ1-2Glc) and laminaribiose (Glcβ1-3Glc), but for steviol glycosides, it only hydrolyzed the C-13/C-19-linked sophorese, instead of the C-13/C-19-linked Glcβ1-2[Glcβ1-3]Glc trisaccharide and Glcβ1-monosaccharide. It efficiently hydrolyzed stevioside (240 g/L) to produce rubusoside (99% yield) at 47.5°C for 70 min. Even when using a crude steviol glycosides extract (500 g/L) containing ∼226 g/L stevioside as the substrate, CsBGL could also convert stevioside to rubusoside (99% yield) at 47.5°C for 2 h, in which the rubusoside concentration increased from the initial 42 g/L to the final 222 g/L. These results reveal that CsBGL would be a promising biocatalyst for the industry-scale production of rubusoside from stevioside or/and the crude steviol glycosides extract. As a natural sweetening and solubilizing agent, rubusoside has great potential in the application of healthy beverages and pharmaceuticals. A novel β-glucosidase CsBGL obtained here from Chryseobacterium scophthalmum 1433 through screening of the environmental microorganisms could specifically hydrolyze the C-13- or/and C-19-linked sophorese of steviol glycosides, but not the C-13- or/and C-19-linked Glcβ1-2[Glcβ1-3]Glc trisaccharide and Glcβ1-monosaccharide of steviol glycosides, and thus could be a promising biocatalyst for efficient rubusoside production from stevioside.
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影响因子：
4.8
作者：
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通讯作者：
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影响因子：
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