Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect
Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect
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DOI：
10.1016/j.bbrc.2011.05.133
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发表时间：
2011-07-01
影响因子：
3.1
通讯作者：
Ishino, Fumitoshi
中科院分区：
文献类型：
--
作者：
Kohda, Takashi;Ogonuki, Narumi;Ishino, Fumitoshi
忠实的转录组调控对于发育非常重要，对于生殖和再生医学的应用也至关重要。胞浆内单精子注射（ICSI）作为人类辅助生殖技术（ART）之一，长期以来一直引起人们对其对发育影响的担忧。然而，尽管过去二十年对 ICSI 和/或体外受精 (IVF) 受孕的孩子进行了许多队列研究，但尚未达成明确的共识。在这项研究中，在严格的条件下对小鼠进行全面的转录组和表型分析，评估了 ICSI 对产前和产后的影响。在这里，我们证明，与 IVF 相比，ICSI 会诱导明显的持久转录组变化，这种变化仍然存在于新生儿阶段。重要的是，在 ICSI 成体中，无论是基因表达还是表型特征都没有观察到显着差异，也没有迹象表明会通过自然交配遗传给下一代。我们的结果表明，ICSI 不会产生终生或跨代影响，但新生儿期 ICSI 的影响仍有待评估。 (C) 2011 Elsevier Inc. 保留所有权利。
Faithful transcriptome regulation is important in development and also crucial for applications in reproductive and regenerative medicine. Intracytoplasmic sperm injection (ICSI), one of the human assisted reproductive technologies (ART), has long raised concerns about its influence on development. No clear consensus has been reached, however, in spite of many cohort studies carried out in the last two decades on the children conceived by ICSI and/or in vitro fertilization (IVF). In this study, the pre- and postnatal effects of ICSI were assessed using comprehensive transcriptome and phenotypic analyses in mice under strict conditions. Here we demonstrate that, in contrast to IVF, ICSI induces distinct long-lasting transcriptome change that remains at the neonatal stage. Importantly, no remarkable differences were observed in the ICSI adults in either the gene expression or phenotypic profiles, and there was no indication of transmission to the next generation via natural mating. Our results suggest there are no lifelong or transgenerational effects of ICSI, but the ICSI effects during neonatal period remain to be evaluated. (C) 2011 Elsevier Inc. All rights reserved.