Chemiluminescence detection for a microchip capillary electrophoresis system fabricated in poly(dimethylsiloxane)
Chemiluminescence detection for a microchip capillary electrophoresis system fabricated in poly(dimethylsiloxane)
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DOI：
10.1021/ac026096s
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发表时间：
2003-01-01
影响因子：
7.4
通讯作者：
Terabe, S
中科院分区：
文献类型：
--
作者：
Liu, BF;Ozaki, M;Terabe, S
采用聚二甲基硅氧烷制作的微芯片毛细管电泳法(MCE)与化学发光检测(CL)相结合，用于化学和生化分析。建立了两个模型化学发光体系：金属离子催化的鲁米诺-过氧化氢反应和丹酚物种共轭过草酸-过氧化氢反应。从灵敏度、重复性和峰对称性三个方面对基于3种芯片模式(交叉、与Y交叉结合、与V交叉结合)的NICE在线化学发光检测的不同策略进行了评估和比较。对于鲁米诺-过氧化氢化学发光体系，Y与Y交叉结合的芯片模式是很有前途的，而对于电渗流不能有效传输CL试剂的过氧草酸盐-过氧化氢体系，交叉结合的芯片模式是首选的。检测下限可达亚微摩尔浓度(中浓度)，重现性好，峰形对称。成功地分离了三种金属离子：铬(III)、钴(II)和铜(II)，并在1min内对丹磺酰苯丙氨酸对映体进行了手性识别，表明MCE与化学发光联用在快速、灵敏分析中具有明显的优势。
Chemiluminescence (CL) detection integrated with a microchip capillary electrophoresis (MCE) system that was fabricated in poly(dimethylsiloxane) was demonstrated for chemical and biochemical analyses. Two model CL systems were involved here: metal ion-catalyzed luminol-peroxide reaction and dansyl species conjugated peroxalate-peroxide reaction. Different strategies based on three chip patterns (cross, cross combining with Y, and cross combining with V) to perform on-line CL detection for NICE were evaluated and compared in terms of sensitivity, reproducibility, and peak symmetry. The chip pattern of cross combining with Y proved to be promising for the luminol-peroxide CL system, while the chip pattern of cross combining with V was preferred for the peroxalate-peroxide system where CL reagent could not be effectively transported by electroosmotic flow. A detection limit down to submicromolar concentrations (mid-attomole) was achieved with good reproducibility and symmetric peak shape. Successful separation of three metal cations such as Cr(III), Co(II), and Cu(II) and chiral recognition of dansyl phenylalanine enantiomers within 1 min revealed distinct advantages of combining MCE with CL detection for rapid and sensitive analyses.