The slice overlay assay: a versatile tool to study the influence of extracellular signals on neuronal development.
The slice overlay assay: a versatile tool to study the influence of extracellular signals on neuronal development.
复制标题
DOI：
10.1126/stke.2002.136.pl9
复制
发表时间：
2002-06-11
期刊：
影响因子：
--
通讯作者：
Ghosh, Anirvan
中科院分区：
文献类型：
--
作者：
Polleux, Franck;Ghosh, Anirvan
We have developed a technique that allows characterization and identification of extracellular signals that regulate various aspects of neuronal differentiation. In this in vitro assay, dissociated cells isolated from the developing cerebral wall are labeled and cultured over organotypic cortical slices. We have used this slice overlay assay to identify some of the extracellular cues that regulate differentiation and patterning of axons and dendrites in the cerebral cortex. This assay can be easily adapted to identify factors that control other aspects of neuronal development, such as proliferation and survival, and can also be used to evaluate the role of extracellular signals in the development of nonneural tissues.
