Thermotropic phase behaviour of α-dipalmitoylphosphatidylcholine multibilayers is influenced to various extents by carotenoids containing different structural features -: evidence from differential scanning calorimetry
Thermotropic phase behaviour of α-dipalmitoylphosphatidylcholine multibilayers is influenced to various extents by carotenoids containing different structural features -: evidence from differential scanning calorimetry
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DOI：
10.1016/s0005-2736(02)00688-0
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发表时间：
2003-01-31
影响因子：
3.4
通讯作者：
Strzalka, K
中科院分区：
文献类型：
--
作者：
Kostecka-Gugala, A;Latowski, D;Strzalka, K
类胡萝卜素是模型膜和天然膜物理性质的有效调节剂。为了证明类胡萝卜素的结构与它们对膜的分子动力学的影响之间的关系，我们研究了五种结构不同的类胡萝卜素的影响：β-胡萝卜素、番茄红素、叶黄素、紫黄质、玉米黄质和另外的胡萝卜素-胡萝卜素的完全饱和衍生物，用差示扫描量热法（DSC）研究了二棕榈酰磷脂酰胆碱（DPPC）多层囊泡的热致相行为。所获得的结果表明，类胡萝卜素的使用调制的多层膜的热致性在不同程度上，拓宽了预过渡和主相变峰，并将它们转移到较低的温度。显着减少的预转变焓（Δ H（p））证明，类胡萝卜素非常强烈地改变其凝胶相的膜特性。几个类胡萝卜素的影响的比较表明，一个刚性的，聚异戊二烯链在改变这种膜的热致性质中起着基本的作用，没有含氧基团的环的存在下，所观察到的相互作用有轻微的意义。类胡萝卜素含有环氧基和/或羟基连接到它们的环修改DPPC多层囊泡的热致相行为强于胡萝卜素-一个结果，它们在DPPC双层中的取向。(C)2002 Elsevier Science B. V.保留所有权利。
Carotenoids are the effective modulators of physical properties of model and natural membranes. To demonstrate the relationship between the structure of carotenoids and their effect on the molecular dynamics of membranes, we have investigated the influence of five structurally different carotenoids: beta-carotene, lycopene, lutein, violaxanthin, zeaxanthin and additionally carotane - a fully saturated derivative of carotene, on thermotropic phase behaviour of dipalmitoylphosphatidylcholine (DPPC) multilamellar vesicles by means of differential scanning calorimetry (DSC). The results obtained indicate that the carotenoids used modulated the thermotropic properties of multibilayers to various extents, broadening the pretransition and the main phase transition peaks and shifting them to lower temperatures. Pronounced decrease of pretransition enthalpy (DeltaH(p)) proves that carotenoids very strongly alter the membrane properties in its gel phase. Comparison of the influence of several carotenoids shows that a rigid, polyisoprenoid chain plays a basic role in altering the thermotropic properties of such membranes and the presence of rings without oxygen-containing groups has a minor significance for the observed interactions. Carotenoids containing epoxy and/or hydroxy groups attached to their rings modify the thermotropic phase behaviour of DPPC multilamellar vesicles stronger than carotenes - a result of their orientation in the DPPC bilayer. (C) 2002 Elsevier Science B.V. All rights reserved.