Sensitive ELISA test for determination of ochratoxin A
Sensitive ELISA test for determination of ochratoxin A
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DOI：
10.1021/jf960442n
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发表时间：
1996-12-01
影响因子：
6.1
通讯作者：
Wolfling, A
中科院分区：
文献类型：
--
作者：
Barna-Vetro, I;Solti, L;Wolfling, A
开发了一种使用单克隆抗体的直接竞争性酶联免疫吸附测定 (ELISA)，用于定量测定不同谷物中的赭曲霉毒素 A (OA)。使用二氯甲烷/柠檬酸混合物提取谷物。事实证明，这种净化程序对于赭曲霉毒素 A 的提取与使用强酸的方案一样有效。标准曲线的平均测定内和测定间变异系数为
A direct, competitive enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody has been developed for quantitative determination of ochratoxin A (OA) in different cereals. A dichloromethane/citric acid mixture was used for extraction of cereals. This cleanup procedure proved to be as effective for ochratoxin A extraction as protocols using strong acids. The mean within-assay and interassay coefficients of variation for the standard curve was