The effect of increased intakes of polyunsaturated fatty acids and vitamin E on DNA damage in human lymphocytes
The effect of increased intakes of polyunsaturated fatty acids and vitamin E on DNA damage in human lymphocytes
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DOI：
10.1096/fasebj.13.15.2138
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发表时间：
1999-12-01
期刊：
影响因子：
4.8
通讯作者：
Duthie, GG
中科院分区：
文献类型：
--
作者：
Jenkinson, AM;Collins, AR;Duthie, GG
研究了增加饲粮中多不饱和脂肪酸（PUFAs）和维生素E对氧化性DNA损伤指标的影响。21名健康男性，年龄28.9 +/- 1.3岁，不吸烟，参加了一项自由生活，分割图/变化试验，其中一半志愿者食用含有5% PUFA作为食物能量的饮食4周，在10周的洗脱期后，再食用15% PUFA的饮食4周。其他志愿者遵循相同的方案，除了他们先食用15%多聚脂肪酸的饮食。志愿者在饮食中分别添加或不添加80毫克/天的α -生育酚醋酸酯；除此之外，总脂肪、碳水化合物、蛋白质和基础维生素E含量保持不变。通过碱性单细胞凝胶电泳（彗星法）检测，200 μ M H2O2诱导的志愿者淋巴细胞DNA损伤和内源性氧化嘧啶形式的DNA损伤，在摄入5% PUFA饮食后显著降低(P
The effect of increasing dietary intakes of polyunsaturated fatty acids (PUFAs) and vitamin E on indices of oxidative DNA damage was investigated. Twenty-one healthy male, nonsmokers aged 28.9 +/- 1.3 years participated in a free-living, split plot/change over trial in which half the volunteers consumed diets containing 5% PUFA as food energy for 4 wk and, after a 10 wk washout period, consumed a 15% PUFA diet for another 4 wk. The other volunteers followed an identical protocol, except that they consumed the 15% PUFA diet first. The diets were provided to volunteers either with or without an additional 80 mg d alpha-tocopherol acetate/day; otherwise total fat, carbohydrates, protein, and basal vitamin E contents remained unchanged. DNA damage induced by 200 mu M H2O2 in lymphocytes from volunteers as well as endogenous DNA damage in the form of oxidized pyrimidines, measured by alkaline single-cell gel electrophoresis (the comet assay), significantly decreased after consumption of the 5% PUFA diet (P